荷兰乌得勒支大学Albert J. R. Heck等研究人员合作发现，超长瞬态可提高基于Orbitrap的单离子质谱仪的灵敏度和分辨率。该项研究成果于2024年3月5日在线发表在《自然—方法学》杂志上。

通过修改Orbitrap超高质量范围质谱仪的数据采集方法，研究人员捕获和监测单个（单一）离子的时间长达25秒，从而使基于Orbitrap的电荷检测质谱仪的信噪比（与1秒相比提高了5倍）、质量分辨率（提高了25倍）以及电荷和质量测定的准确性得到了相应的大幅提高。

据悉，基于Orbitrap的电荷检测质谱法利用单分子灵敏度对高度异质、大质量的大分子组装体进行质量分析。

附：英文原文

Title: Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry

Author: Deslignire, Evolne, Yin, Victor C., Ebberink, Eduard H. T. M., Rolland, Amber D., Barendregt, Arjan, Wrner, Tobias P., Nagornov, Konstantin O., Kozhinov, Anton N., Fort, Kyle L., Tsybin, Yury O., Makarov, Alexander A., Heck, Albert J. R.

Issue&Volume: 2024-03-05

Abstract: Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1–2s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.

DOI: 10.1038/s41592-024-02207-8

Source: https://www.nature.com/articles/s41592-024-02207-8