武汉大学周翔团队报道了链亲和素-生物素相互作用调控CRISPR/Cas9 †。相关研究成果于2024年2月29日发表在国际顶尖学术期刊《中国化学》。

目前，CRISPR/Cas9技术已在各个领域得到广泛应用。然而，CRISPR/Cas9的实用性受到与其可靠性和安全性有关的问题的阻碍，这些问题主要源于系统的不受控制的活动。因此，设计和开发具有可控活性的CRISPR/Cas9系统至关重要。

具有小分子量生物素和具有显著空间位阻特征的链亲和素，由于它们的相互作用特性，可以作为理想的关闭开关（称为“生物活性制动器”）。

该文中，研究人员提出了一种策略，将链亲和素-生物素相互作用作为CRISPR/Cas9的“制动系统”，有效地关闭CRISPR/Cas9的酶活性。

附：英文原文

Title: Regulating CRISPR/Cas9 Using Streptavidin-Biotin Interactions†

Author: Wei Shen, Wei Xiong, Qianqian Qi, Xingyu Liu, Zhongpao Xie, Yuanyuan Zhang, Jinxuan Hou, Tian Tian, Xiang Zhou

Issue&Volume: 2024-02-29

Abstract: Currently, CRISPR/Cas9 technology has found widespread applications across various domains. However, the utility of CRISPR/Cas9 is encumbered by issues pertaining to its reliability and safety, primarily stemming from the uncontrolled activity of the system. Therefore, the design and development of CRISPR/Cas9 systems with controllable activity is of paramount importance. Biotin, characterized by its small molecular weight, and streptavidin, distinguished by its substantial spatial steric hindrance, can be harnessed as an ideal OFF switch (termed a "bioactivity brake") due to their interaction characteristics. In this work, we present a strategy that employs the streptavidin-biotin interaction as a "brake system" for CRISPR/Cas9, effectively allowing for the shutdown of the enzymatic activity of CRISPR/Cas9.

DOI: 10.1002/cjoc.202300662

Source: https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202300662