德国自由柏林大学Helge Ewers团队开发出可控释放单分子的光遗传学方法。2024年3月8日,国际知名学术期刊《自然—方法学》在线发表了这一成果。
附:英文原文
Title: An optogenetic method for the controlled release of single molecules
Author: Kashyap, Purba, Bertelli, Sara, Cao, Fakun, Kostritskaia, Yulia, Blank, Fenja, Srikanth, Niranjan A., Schlack-Leigers, Claire, Saleppico, Roberto, Bierhuizen, Dolf, Lu, Xiaocen, Nickel, Walter, Campbell, Robert E., Plested, Andrew J. R., Stauber, Tobias, Taylor, Marcus J., Ewers, Helge
Issue&Volume: 2024-03-08
Abstract: We developed a system for optogenetic release of single molecules in cells. We confined soluble and transmembrane proteins to the Golgi apparatus via a photocleavable protein and released them by short pulses of light. Our method allows for a light dose-dependent delivery of functional proteins to the cytosol and plasma membrane in amounts compatible with single-molecule imaging, greatly simplifying access to single-molecule microscopy of any protein in live cells. We were able to reconstitute ion conductance by delivering BK and LRRC8/volume-regulated anion channels to the plasma membrane. Finally we were able to induce NF-kB signaling in Tlymphoblasts stimulated by interleukin-1 by controlled release of a signaling protein that had been knocked out. We observed light-induced formation of functional inflammatory signaling complexes that triggered phosphorylation of the inhibitor of nuclear factor kappa-B kinase only in activated cells. We thus developed an optogenetic method for the reconstitution and investigation of cellular function at the single-molecule level.
DOI: 10.1038/s41592-024-02204-x
Source: https://www.nature.com/articles/s41592-024-02204-x
Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:47.99
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex