瑞典卡罗林斯卡学院Ning Xu Landén等研究人员合作绘制出，人类皮肤创伤愈合的时空单细胞路线图。这一研究成果于2024年12月26日在线发表在国际学术期刊《细胞—干细胞》上。

研究人员表示，创伤愈合对人类健康至关重要，但人类创伤修复中的细胞动态和协调机制仍未得到充分探讨。

为了解决这一问题，研究人员通过单细胞多组学分析对人类皮肤创伤组织进行了研究。该研究涵盖了从同一患者的炎症、增生和重塑阶段的创伤修复过程，前所未有地在时空分辨率上，监测了人类皮肤创伤愈合的细胞和分子动态。这一独特的路线图揭示了创伤边缘的细胞结构，并确定FOSL1作为再上皮化的关键驱动因子。

结果显示，促炎性巨噬细胞和成纤维细胞像接力赛一样，在不同的愈合阶段依次支持角质形成细胞的迁移。通过与静脉溃疡和糖尿病足溃疡的单细胞数据进行比较，研究人员揭示了慢性创伤中，角质形成细胞迁移失败与炎症反应受损之间的联系。此外，通过比较人类和小鼠急性创伤的转录组，研究人员强调了该路线图，在将基础研究与临床创新相结合中的不可或缺的价值。

附：英文原文

Title: Spatiotemporal single-cell roadmap of human skin wound healing

Author: Zhuang Liu, Xiaowei Bian, Lihua Luo, sa K. Bjrklund, Li Li, Letian Zhang, Yongjian Chen, Lei Guo, Juan Gao, Chunyan Cao, Jiating Wang, Wenjun He, Yunting Xiao, Liping Zhu, Karl Annusver, Nusayhah Hudaa Gopee, Daniela Basurto-Lozada, David Horsfall, Clare L. Bennett, Maria Kasper, Muzlifah Haniffa, Pehr Sommar, Dongqing Li, Ning Xu Landén

Issue&Volume: 2024-12-26

Abstract: Wound healing is vital for human health, yet the details of cellular dynamics and coordination in human wound repair remain largely unexplored. To address this, we conducted single-cell multi-omics analyses on human skin wound tissues through inflammation, proliferation, and remodeling phases of wound repair from the same individuals, monitoring the cellular and molecular dynamics of human skin wound healing at an unprecedented spatiotemporal resolution. This singular roadmap reveals the cellular architecture of the wound margin and identifies FOSL1 as a critical driver of re-epithelialization. It shows that pro-inflammatory macrophages and fibroblasts sequentially support keratinocyte migration like a relay race across different healing stages. Comparison with single-cell data from venous and diabetic foot ulcers uncovers a link between failed keratinocyte migration and impaired inflammatory response in chronic wounds. Additionally, comparing human and mouse acute wound transcriptomes underscores the indispensable value of this roadmap in bridging basic research with clinical innovations.

DOI: 10.1016/j.stem.2024.11.013

Source: https://www.cell.com/cell-stem-cell/abstract/S1934-5909(24)00412-0