北京大学药学院谢海燕团队报道，“AND门”逻辑Frster共振能量转移/磁共振调谐纳米探针，可编程抗肿瘤免疫成像。相关研究成果发表在2024年11月6日出版的《美国化学会杂志》。

同时检测与时空动态免疫事件相关的不同生物标志物，对于准确评估抗肿瘤免疫效果尤为重要。

该文中，研究人员开发了一种AND门逻辑双共振能量转移纳米探针（称为DRET），用于动态监测程序化CD8+T细胞活化和肿瘤细胞凋亡。免疫治疗诱导CD8+T细胞分泌颗粒酶B，随后凋亡肿瘤细胞释放半胱氨酸天冬氨酸蛋白酶-3，分别激活“与门”逻辑DRET的一层。

由此产生的荧光恢复和磁共振T1增强，可用于精确的免疫调节药物筛选、早期疗效预测和免疫分层。特别是，不仅可以将“应答者”与“无应答者”区分开来，还可以从“维持应答者”中识别出“获得性耐药性”，为准确评估抗肿瘤免疫提供了一种新方法。

附：英文原文

Title: AND-Gate Logic Frster Resonance Energy Transfer/Magnetic Resonance Tuning Nanoprobe for Programmable Antitumor Immunity Imaging

Author: Xianbin Ma, Mingchuan Mao, Zhenya Liu, Chao Liang, Jiaqi He, Yun Qu, Luzheng Xu, Ran Cheng, Wanru Zhuang, Yao Lei, Weidong Nie, Lan Yuan, Dai-Wen Pang, Hai-Yan Xie

Issue&Volume: November 6, 2024

Abstract: Simultaneous detection of different biomarkers related to the spatiotemporally dynamic immune events is of particular importance for the accurate evaluation of antitumor immune effects. Here, we have developed an AND-gate logic dual resonance energy transfer nanoprobe (named DRET) for dynamic monitoring of programmed CD8+ T cell activation and tumor cell apoptosis. Immunotherapy-induced granzyme B secretion from CD8+ T cells and the subsequent caspase-3 release from apoptotic tumor cells individually activate one of the tiers of the “AND-gate” logic DRET. The resulting fluorescence recovery and magnetic resonance T1 enhancement can be used for precise immunomodulatory drug screening, early efficacy prediction, and immune stratification. Particularly, not only “Responders” can be distinguished from “Non-responders”, but also “Acquired resistance” can be identified from “Maintain responders”, providing a novel approach to put forward the accurate evaluation of antitumor immunity.

DOI: 10.1021/jacs.4c11072

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.4c11072