东北林业大学金丽华等研究人员合作发现，Rab1和Syntaxin 17通过果蝇β-整合素运输调节造血稳态。2024年11月13日，《遗传学报》杂志在线发表了这一最新研究成果。

研究人员探讨了Rab1功能障碍对循环血细胞和淋巴腺细胞中β-整合素转运的影响。Rab1损伤破坏了β-整合素的内体转运，导致其在细胞膜上的异常定位，这促进了层粒细胞的分化，并分别改变了循环血细胞和淋巴腺中前体细胞的动力学。

研究人员还发现，β-整合素的错误定位依赖于黏附蛋白DE-钙粘蛋白。在后部信号中心（PSC）细胞的细胞边界上，β-整合素的减少导致了PSC细胞数量的减少和层粒细胞的分化。此外，Rab1通过Q-SNARE蛋白Syntaxin 17（Syx17）调节β-整合素的转运。

这些研究结果表明，Rab1和Syx17在不同的造血区室中调节β-整合素的不同转运途径，并维持果蝇的造血稳态。

据悉，造血对机体健康至关重要，而果蝇作为一种有效的遗传模型，因其与脊椎动物的调控机制相似而被广泛使用。在幼虫中，造血主要发生在淋巴腺中，淋巴腺包含不同的区域，包括皮质区、中间区、髓质区和PSC。Rab1对于膜转运和维持细胞黏附分子的定位至关重要，但其在造血稳态中的作用尚未完全理解。

附：英文原文

Title: Rab1 and Syntaxin 17 regulate hematopoietic homeostasis through β-integrin trafficking in Drosophila

Author: Shichao Yu a, Li Hua Jin a

Issue&Volume: 2024/11/13

Abstract: Hematopoiesis is crucial for organismal health, and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with vertebrates. In larvae, hematopoiesis primarily occurs in the lymph gland, which contains distinct zones, including the cortical zone, intermediate zone, medullary zone, and posterior signaling center (PSC). Rab1 is vital for membrane trafficking and maintaining the localization of cell adhesion molecules, yet its role in hematopoietic homeostasis is not fully understood. This study investigates the effects of Rab1 dysfunction on β-integrin trafficking within circulating hemocytes and lymph gland cells. Rab1 impairment disrupts the endosomal trafficking of β-integrin, leading to its abnormal localization on cell membranes, which promotes lamellocyte differentiation and altered progenitor dynamics in circulating hemocytes and lymph glands, respectively. We also show that the mislocalization of β-integrin was dependent on the adhesion protein DE-cadherin. The reduction of β-integrin at cell boundaries in PSC cells leads to fewer PSC cells and lamellocyte differentiation. Furthermore, Rab1 regulates the trafficking of β-integrin via the Q-SNARE protein Syntaxin 17 (Syx17). Our findings indicate that Rab1 and Syx17 regulate distinct trafficking pathways for β-integrin in different hematopoietic compartments and maintain hematopoietic homeostasis of Drosophila.

DOI: 10.1016/j.jgg.2024.11.001

Source: https://www.sciencedirect.com/science/article/abs/pii/S1673852724002947