美国陈-扎克伯格生物中心Loïc A. Royer等研究人员合作发现，一份多模态斑马鱼发育图谱揭示晚脊椎动物多能轴向祖细胞的状态转变动态。2024年10月24日，国际知名学术期刊《细胞》在线发表了这一成果。

研究人员介绍了Zebrahub，这是一个动态的斑马鱼胚胎发育图谱，整合了单细胞测序时间序列数据和通过光片显微镜辅助的谱系重建。该图谱通过对十个发育阶段的单个胚胎进行测序，以及对细胞轨迹的重建，提供了对斑马鱼发育的高分辨率和深入的分子见解。

Zebrahub还包含一个互动工具，用于导航源自光片显微镜数据的复杂细胞流动和谱系，从而实现计算命运追踪实验。为了展示该多模态资源的多样性，研究人员利用Zebrahub提供了关于神经中胚层祖细胞（NMP）多能性和联合肾脏-血管母细胞祖细胞群体起源的新见解。

研究人员表示，阐明生物体发育过程需要全面了解空间、时间和分子领域的细胞谱系。

附：英文原文

Title: A multimodal zebrafish developmental atlas reveals the state-transition dynamics of late-vertebrate pluripotent axial progenitors

Author: Merlin Lange, Alejandro Granados, Shruthi VijayKumar, Jordo Bragantini, Sarah Ancheta, Yang-Joon Kim, Sreejith Santhosh, Michael Borja, Hirofumi Kobayashi, Erin McGeever, Ahmet Can Solak, Bin Yang, Xiang Zhao, Yang Liu, Angela M. Detweiler, Sheryl Paul, Ilan Theodoro, Honey Mekonen, Chris Charlton, Tiger Lao, Rachel Banks, Sheng Xiao, Adrian Jacobo, Keir Balla, Kyle Awayan, Samuel D’Souza, Robert Haase, Alexandre Dizeux, Olivier Pourquie, Rafael Gómez-Sjberg, Greg Huber, Mattia Serra, Norma Neff, Angela Oliveira Pisco, Loc A. Royer

Issue&Volume: 2024-10-24

Abstract: Elucidating organismal developmental processes requires a comprehensive understanding of cellular lineages in the spatial, temporal, and molecular domains. In this study, we introduce Zebrahub, a dynamic atlas of zebrafish embryonic development that integrates single-cell sequencing time course data with lineage reconstructions facilitated by light-sheet microscopy. This atlas offers high-resolution and in-depth molecular insights into zebrafish development, achieved through the sequencing of individual embryos across ten developmental stages, complemented by reconstructions of cellular trajectories. Zebrahub also incorporates an interactive tool to navigate the complex cellular flows and lineages derived from light-sheet microscopy data, enabling in silico fate-mapping experiments. To demonstrate the versatility of our multimodal resource, we utilize Zebrahub to provide fresh insights into the pluripotency of neuro-mesodermal progenitors (NMPs) and the origins of a joint kidney-hemangioblast progenitor population.

DOI: 10.1016/j.cell.2024.09.047

Source: https://www.cell.com/cell/abstract/S0092-8674(24)01147-4