江苏省农业科学院赵涵研究组发现，时间序列转录组分析揭示在调控玉米对氮敏感性中起作用的转录因子。相关论文于2024年10月10日在线发表于国际学术期刊《遗传学报》。

据悉，氮（N）既是植物的重要常量营养元素，又是一个信号分子。揭示参与氮代谢的关键调控因子有助于解析氮代谢机制，这对于培育具有高氮利用效率的玉米至关重要。

研究人员表示，玉米的两个品系B73和Ki11在氯酸盐和低氮耐受性上表现出差异。时间序列转录组分析显示，B73和Ki11中的氮调控基因（NUG）在硝酸盐变化下有不同的响应模式。

通过共表达网络分析，B73和Ki11在氮响应模块数量和转录因子（TF）调控网络上表现出显著差异。B73中有23个独特的转录因子，而Ki11中有41个。MADS26是B73氮响应网络中的一个独特转录因子，其在B73和Ki11中的表达水平和氮响应模式不同。

MADS26的过表达增强了玉米对氯酸盐的敏感性以及对硝酸盐的利用，至少部分解释了B73和Ki11之间氯酸盐耐受性和低氮敏感性的差异。该研究为减少氮过度使用的玉米育种，提供了独特的见解和有前景的候选基因。

附：英文原文

Title: Time-course transcriptomic analysis reveals transcription factors involved in modulating nitrogen sensibility in maize

Author: Han Zhao a

Issue&Volume: 2024/10/10

Abstract: Nitrogen (N) serves both as a vital macronutrient and a signaling molecule for plants. Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism, which is essential for developing maize with high N use efficiency. Two maize lines, B73 and Ki11, show differential chlorate and low-N tolerance. Time-course transcriptomic analysis reveals that the expression of NUGs in B73 and Ki11 have distinct responsive patterns to nitrate variation. By the coexpression networks, significant differences in the number of N response modules and regulatory networks of transcription factors (TFs) are revealed between B73 and Ki11. There are 23 unique TFs in B73 and 41 unique TFs in Ki11. MADS26 is a unique TF in the B73 N response network, with different expression level and N response pattern in B73 and Ki11. Overexpression of MADS26 enhances the sensitivity to chlorate and the utilization of nitrate in maize, at least partially explaining the differential chlorate tolerance and Low-N sensitivity between B73 and Ki11. The findings in this work provide unique insights and promising candidates for maize breeding to reduce unnecessary N overuse.

DOI: 10.1016/j.jgg.2024.09.021

Source: https://www.sciencedirect.com/science/article/pii/S1673852724002595