中国科学院东北地理所农业技术中心夏正俊等研究人员合作发现，PvPW1编码的β-1,3-葡聚糖酶9中的一个单核苷酸多态性与菜豆的豆荚宽度相关。2024年10月9日，《遗传学报》在线发表了这项成果。

研究人员表示，豆荚宽度影响着荚果的大小、形状、产量和消费者偏好。

研究人员通过重组植物的基因分型和表型分析，绘制了与荚果宽度相关的定量性状位点PvPW1。研究人员确定了编码β-1,3-葡聚糖酶9蛋白的基因Phvul.006G072800是PvPW1的致病基因。分析显示，PvPW1G3555等位基因能够正向调节荚果宽度，在17个双亲F2群体中，荚果宽度表型与PvPW1G3555C基因型之间的关联分析揭示了这一点。133个宽荚品种中有97.7%携带PvPW1G3555，而78个窄荚品种中有82.1%携带PvPW1C3555，这表明在普通豆育种过程中对PvPW1施加了强选择压力。

从59个普通豆品种的重新测序数据中，研究人员识别出与PvPW1C3555相关的内含子中的8个碱基缺失，导致了PvM436的InDel标记的开发。使用PvM436对317个普通豆品种进行基因分型的结果表明，携带PvM436247和PvM436227等位基因的品种相比于携带PvM436219等位基因的品种具有更宽的豆荚。这确立了PvM436作为荚果育种分子标记的可靠性。这些发现强调了PvPW1作为调节豆荚宽度的关键基因，并突出了PvM436在荚果育种中作为标记辅助选择的应用价值。

附：英文原文

Title: A single-nucleotide polymorphism in PvPW1 encoding β-1,3-glucanase 9 is associated with pod width in Phaseolus vulgaris L.

Author: Jing Wu, Dajun Liu, Yanhua Li, Zhengjun Xia

Issue&Volume: 2024/10/09

Abstract: Pod width influences pod size, shape, yield, and consumer preference in snap beans (Phaseolus vulgaris L.). In this study, we map PvPW1, a quantitative trait locus associated with pod width in snap beans, through genotyping and phenotyping of recombinant plants. We identify Phvul.006G072800, encoding the β-1,3-glucanase 9 protein, as the causal gene for PvPW1. The PvPW1G3555 allele is found to positively regulate pod width, as revealed by an association analysis between pod width phenotype and the PvPW1G3555C genotype across 17 bi-parental F2 populations. 97.7% of the 133 wide pod accessions carry PvPW1G3555, while 82.1% of the 78 narrow pod accessions carry PvPW1C3555, indicating strong selection pressure on PvPW1 during common bean breeding. Re-sequencing data from 59 common bean cultivars identify an 8-bp deletion in the intron linked to PvPW1C3555, leading to the development of the InDel marker of PvM436. Genotyping 317 common bean accessions with PvM436 demonstrated that accessions with PvM436247 and PvM436227 alleles have wider pods compared to those with PvM436219 allele, establishing PvM436 as a reliable marker for molecular breeding in snap beans. These findings highlight PvPW1 as a critical gene regulating pod width and underscore the utility of PvM436 in marker-assisted selection for snap bean breeding.

DOI: 10.1016/j.jgg.2024.09.020

Source: https://www.sciencedirect.com/science/article/pii/S1673852724002583