近日，法国马赛大学的Loic LeGoff&Anne Sentenac及其研究团队取得一项新进展。经过不懈努力，他们利用扩展景深随机照明显微镜（EDF-RIM）实现超分辨率投影成像。相关研究成果已于2024年10月10日在国际知名学术期刊《光：科学与应用》上发表。

该研究团队在随机照明显微镜（RIM）中实施了扩展景深（EDF）技术。RIM采用多点斑状照明和方差数据处理，可将分辨率提高一倍。该技术尤其适用于厚样本的成像，因为它无需了解照明模式。研究人员展示了生物组织和细胞的高分辨率投影图像。

与采用传统二维RIM对成像体积进行顺序扫描相比，EDF-RIM在速度上提升了一个数量级，同时降低了光剂量，而分辨率则保持相当。由于EDF模式下会丢失轴向信息，研究人员针对以细胞层形式组织的样本，提出了一种恢复样本形貌的方法。

据悉，荧光显微镜的最终目标是实现对日益增大的生物样本的高分辨率成像。当沿光轴的信息压缩不妨碍图像解释时，扩展景深便为加速大型样本成像提供了一种潜在的解决方案。

附：英文原文

Title: Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging

Author: Mazzella, Lorry, Mangeat, Thomas, Giroussens, Guillaume, Rogez, Benoit, Li, Hao, Creff, Justine, Saadaoui, Mehdi, Martins, Carla, Bouzignac, Ronan, Labouesse, Simon, Idier, Jrome, Galland, Frdric, Allain, Marc, Sentenac, Anne, LeGoff, Loic

Issue&Volume: 2024-10-10

Abstract: The ultimate aim of fluorescence microscopy is to achieve high-resolution imaging of increasingly larger biological samples. Extended depth of field presents a potential solution to accelerate imaging of large samples when compression of information along the optical axis is not detrimental to the interpretation of images. We have implemented an extended depth of field (EDF) approach in a random illumination microscope (RIM). RIM uses multiple speckled illuminations and variance data processing to double the resolution. It is particularly adapted to the imaging of thick samples as it does not require the knowledge of illumination patterns. We demonstrate highly-resolved projective images of biological tissues and cells. Compared to a sequential scan of the imaged volume with conventional 2D-RIM, EDF-RIM allows an order of magnitude improvement in speed and light dose reduction, with comparable resolution. As the axial information is lost in an EDF modality, we propose a method to retrieve the sample topography for samples that are organized in cell sheets.

DOI: 10.1038/s41377-024-01612-0

Source: https://www.nature.com/articles/s41377-024-01612-0