中国科学技术大学Xing Liu课题组发现，Aurora B促进CENP-T-CENP-W相互作用来引导有丝分裂中染色体的准确分离。这一研究成果于2024年1月10日在线发表在国际学术期刊《分子细胞生物学报》上。

研究人员表示，有丝分裂过程中染色体的准确分离取决于连接中心染色质和纺锤体微管的动点。在染色体分离过程中，中心粒通过动粒构成的中心粒相关网络（CCAN）被单个微管捕获。CCAN包含16个亚基，其中包括CENP-W和CENP-T。然而，CCAN组装的分子识别和有丝分裂调控仍然难以捉摸。

研究人员发现CENP-W与组蛋白折叠结构域和CENP-T的一个未表征的N端区域结合。Aurora B使CENP-W在Thr60处磷酸化，从而增强了CENP-W和CENP-T之间的相互作用，以确保有丝分裂中稳健的分裂后期染色体排列和准确的染色体分离。这些发现描述了一个保守的信号级联，它将蛋白质磷酸化与CCAN的完整性结合起来，从而维持基因组的稳定性。

附：英文原文

Title: Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis

Author: Liu, Wei, Dou, Zhen, Wang, Chunyue, Zhao, Gangyin, Wu, Fengge, Wang, Chunli, Aikhionbare, Felix, Ye, Mingliang, Sedzro, Divine Mensah, Yang, Zhenye, Fu, Chuanhai, Wang, Zhikai, Gao, Xinjiao, Yao, Xuebiao, Song, Xiaoyu, Liu, Xing

Issue&Volume: 2024-01-10

Abstract: Accurate chromosome segregation in mitosis depends on kinetochores that connect centromeric chromatin to spindle microtubules. Centromeres are captured by individual microtubules via a kinetochore constitutive centromere-associated network (CCAN) during chromosome segregation. CCAN contains 16 subunits, including CENP-W and CENP-T. However, the molecular recognition and mitotic regulation of the CCAN assembly remain elusive. Here, we revealed that CENP-W binds to the histone fold domain and an uncharacterized N-terminal region of CENP-T. Aurora B phosphorylates CENP-W at Thr60, which enhances the interaction between CENP-W and CENP-T to ensure robust metaphase chromosome alignment and accurate chromosome segregation in mitosis. These findings delineate a conserved signaling cascade that integrates protein phosphorylation with CCAN integrity for the maintenance of genomic stability.

DOI: 10.1093/jmcb/mjae001

Source: https://dx.doi.org/10.1093/jmcb/mjae001