北京大学医学部Yuxin Yin团队近期取得重要工作进展，他们研究提出，来自PTEN的CircPTEN-MT调节线粒体能量代谢。相关研究成果2024年1月5日在线发表于《遗传学报》杂志上。

据介绍，PTEN是一种多功能基因，参与多种生理和病理过程。环状RNA（circRNA）是由mRNA加工过程中的反向剪接事件产生的，并通过与RNA或蛋白质结合参与细胞生物学过程。然而，PTEN相关的circRNA在很大程度上是未知的。

研究人员报道了circPTEN MT（hsa_cir_0002934）是一种由PTEN外显子3、4和5编码的环状RNA，是线粒体能量代谢的关键调节因子。CircPTEN MT定位于线粒体，并与富含亮氨酸的五肽重复序列含蛋白（LRPPRC）物理相关，该蛋白调节线粒体中转录后基因的表达。敲低circPTEN MT可减少LRPPRC和SRA茎环相互作用RNA结合蛋白（SLIRP）的相互作用，并抑制线粒体mRNA的多腺苷酸化，从而降低线粒体复合物I亚基的mRNA水平，并减少线粒体膜电位（MMP）和ATP的产生。

总之，这一数据表明，circPTEN MT是细胞能量代谢的重要调节因子。这项研究扩展了人们对PTEN作用的理解，PTEN产生具有不同和独立功能的线性和环状RNA。

附：英文原文

Title: CircPTEN-MT from PTEN regulates mitochondrial energy metabolism

Author: anonymous

Issue&Volume: 2024/01/05

Abstract: PTEN is a multifunctional gene that is involved in a variety of physiological and pathological processes. Circular RNAs (circRNAs) are generated from back-splicing events during mRNA processing and participate in cell biological processes through binding to RNAs or proteins. However, PTEN-related circRNAs are largely unknown. Here we report that circPTEN-MT (hsa_circ_0002934) is a circular RNA encoded by exons 3, 4, and 5 of PTEN and is a critical regulator of mitochondrial energy metabolism. CircPTEN-MT is localized to mitochondria and physically associated with leucine-rich pentatricopeptide repeat-containing protein (LRPPRC), which regulates posttranscriptional gene expression in mitochondria. Knocking down circPTEN-MT reduces the interaction of LRPPRC and SRA stem-loop interacting RNA binding protein (SLIRP) and inhibits the polyadenylation of mitochondrial mRNA, which decreases the mRNA level of the mitochondrial complex Ι subunit and reduces mitochondrial membrane potential (MMP) and ATP production. Our data demonstrate that circPTEN-MT is an important regulator of cellular energy metabolism. This study expands our understanding of the role of PTEN which produces both linear and circular RNAs with different and independent functions.

DOI: 10.1016/j.jgg.2023.12.011

Source: https://www.sciencedirect.com/science/article/abs/pii/S1673852724000018