复旦大学Yuanting Zheng，Leming Shi，Feng Qian，中国医学科学院北京医院Rui Zhang和美国食品药品管理局Joshua Xu共同合作，近期取得重要工作进展。他们研究利用Quartet RNA参考材料通过基于比率的分析提高了转录组数据的质量。相关研究成果2023年9月7日在线发表于《自然—生物技术》杂志上。

据介绍，经认证的RNA参考材料对于评估RNA测序的可靠性是必不可少的，以检测临床环境中固有的微小生物学差异，例如疾病的分子亚型。

作为多组学分析的质量控制和数据整合的Quartet项目的一部分，研究人员建立了四种RNA参考材料，这些材料来源于单卵双胞胎家族四个成员的永生B淋巴母细胞系。此外，在两个样本之间构建了基于比率的转录组范围参考数据集，提供了跨平台和跨实验室的“基本事实”。对Quartet样本之间内在生物学差异的研究能够在比率水平上对转录组学测量的跨批次整合进行敏感评估。

总之，Quartet RNA参考材料与基于比率的参考数据集相结合，可以作为评估和提高临床和生物学环境中转录组数据质量的独特资源。

附：英文原文

Title: Quartet RNA reference materials improve the quality of transcriptomic data through ratio-based profiling

Author: Yu, Ying, Hou, Wanwan, Liu, Yaqing, Wang, Haiyan, Dong, Lianhua, Mai, Yuanbang, Chen, Qingwang, Li, Zhihui, Sun, Shanyue, Yang, Jingcheng, Cao, Zehui, Zhang, Peipei, Zi, Yi, Liu, Ruimei, Gao, Jian, Zhang, Naixin, Li, Jingjing, Ren, Luyao, Jiang, He, Shang, Jun, Zhu, Sibo, Wang, Xiaolin, Qing, Tao, Bao, Ding, Li, Bingying, Li, Bin, Suo, Chen, Pi, Yan, Wang, Xia, Dai, Fangping, Scherer, Andreas, Mattila, Pirkko, Han, Jinxiong, Zhang, Lijun, Jiang, Hui, Thierry-Mieg, Danielle, Thierry-Mieg, Jean, Xiao, Wenming, Hong, Huixiao, Tong, Weida, Wang, Jing, Li, Jinming, Fang, Xiang, Jin, Li, Xu, Joshua, Qian, Feng, Zhang, Rui, Shi, Leming, Zheng, Yuanting

Issue&Volume: 2023-09-07

Abstract: Certified RNA reference materials are indispensable for assessing the reliability of RNA sequencing to detect intrinsically small biological differences in clinical settings, such as molecular subtyping of diseases. As part of the Quartet Project for quality control and data integration of multi-omics profiling, we established four RNA reference materials derived from immortalized B-lymphoblastoid cell lines from four members of a monozygotic twin family. Additionally, we constructed ratio-based transcriptome-wide reference datasets between two samples, providing cross-platform and cross-laboratory ‘ground truth’. Investigation of the intrinsically subtle biological differences among the Quartet samples enables sensitive assessment of cross-batch integration of transcriptomic measurements at the ratio level. The Quartet RNA reference materials, combined with the ratio-based reference datasets, can serve as unique resources for assessing and improving the quality of transcriptomic data in clinical and biological settings. A new RNA reference set improves detection of differential expression in clinical settings.

DOI: 10.1038/s41587-023-01867-9

Source: https://www.nature.com/articles/s41587-023-01867-9