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无酶催化DNA线路驱动的组成动态网络的级联反馈驱动和空间局部出现
作者:小柯机器人 发布时间:2023/6/2 16:08:44

东南大学张袁健团队报道了无酶催化DNA线路驱动的组成动态网络的级联、反馈驱动和空间局部出现。相关研究成果于2023年5月31日发表在《美国化学会杂志》。

无酶催化发夹组装(CHA)工艺被引入作为一种功能反应模块,用于从一组核酸中引导、高通量、出现和进化组成动态网络(CDN)。该过程用于组装具有可变复杂性、功能性和空间限制的网络,该系统为生命起源前的动态网络的进化提供了可能的机制途径。将一组四个或六个结构工程发夹置于启动子P1下,分别导致CHA引导的[2×2]CDN的出现或[3×3]CDN的进化。一组支链三臂DNA发夹功能化连接与启动子链的反应激活了CHA诱导的模拟天然基因调控网络的三维(3D)CDN框架的出现。研究人员还演示了双层CHA级联路线或交叉催化CHA路线的激活,以及CDN的级联驱动反馈驱动进化。

此外,研究人员将四发夹修饰的DNA四面体纳米结构置于辅助启动子链中,模拟了动态平衡的基于DNA四面体的CDN的进化,该CDN经历二次燃料动态重构。最后,利用DNA四面体结构对细胞的有效渗透,研究人员将四个发夹功能化四面体反应模块整合到细胞中。空间定位的miRNA触发了CHA进化和CDNs的重新配置,使细胞内RNA的逻辑门控成像成为可能。除了这些系统的生物分析应用之外,该研究还介绍了生命起源前功能网络进化的可能机制途径。

附:英文原文

Title: Cascaded, Feedback-Driven, and Spatially Localized Emergence of Constitutional Dynamic Networks Driven by Enzyme-Free Catalytic DNA Circuits

Author: Zhixin Zhou, Nina Lin, Yu Ouyang, Songqin Liu, Yuanjian Zhang, Itamar Willner

Issue&Volume: May 31, 2023

Abstract: The enzyme-free catalytic hairpin assembly (CHA) process is introduced as a functional reaction module for guided, high-throughput, emergence, and evolution of constitutional dynamic networks, CDNs, from a set of nucleic acids. The process is applied to assemble networks of variable complexities, functionalities, and spatial confinement, and the systems provide possible mechanistic pathways for the evolution of dynamic networks under prebiotic conditions. Subjecting a set of four or six structurally engineered hairpins to a promoter P1 leads to the CHA-guided emergence of a [2 × 2] CDN or the evolution of a [3 × 3] CDN, respectively. Reacting of a set of branched three-arm DNA-hairpin-functionalized junctions to the promoter strand activates the CHA-induced emergence of a three-dimensional (3D) CDN framework emulating native gene regulatory networks. In addition, activation of a two-layer CHA cascade circuit or a cross-catalytic CHA circuit and cascaded driving feedback-driven evolution of CDNs are demonstrated. Also, subjecting a four-hairpin-modified DNA tetrahedron nanostructure to an auxiliary promoter strand simulates the evolution of a dynamically equilibrated DNA tetrahedron-based CDN that undergoes secondary fueled dynamic reconfiguration. Finally, the effective permeation of DNA tetrahedron structures into cells is utilized to integrate the four-hairpin-functionalized tetrahedron reaction module into cells. The spatially localized miRNA-triggered CHA evolution and reconfiguration of CDNs allowed the logic-gated imaging of intracellular RNAs. Beyond the bioanalytical applications of the systems, the study introduces possible mechanistic pathways for the evolution of functional networks under prebiotic conditions.

DOI: 10.1021/jacs.3c02083

Source: https://pubs.acs.org/doi/10.1021/jacs.3c02083

期刊信息

JACS:《美国化学会志》,创刊于1879年。隶属于美国化学会,最新IF:16.383
官方网址:https://pubs.acs.org/journal/jacsat
投稿链接:https://acsparagonplus.acs.org/psweb/loginForm?code=1000