武汉大学何光存团队发现，一个三方变阻器控制宿主植物对昆虫的自我调节抗性。2023年6月14日，国际知名学术期刊《自然》发表了这一成果。

研究人员表示，植物部署受体样激酶和核苷酸结合的富含亮氨酸重复受体来赋予食草动物宿主植物抗性（HPR）。昆虫和它们的宿主之间的这些基因对基因的相互作用已被提出50多年。然而，支撑HPR的分子和细胞机制一直难以捉摸，因为昆虫无毒性效应物的身份和感应机制一直是未知的。

研究人员确定了一个由植物免疫受体感知的昆虫唾液蛋白。褐飞虱（Nilaparvata lugens Stål）的BPH14交互唾液蛋白（BISP）在进食时分泌到水稻（Oryza sativa）中。在易感植物中，BISP针对O. satvia RLCK185（OsRLCK185；以下用Os表示O. satvia相关的蛋白质或基因），来抑制基础防御。在抗性植物中，核苷酸结合的富含亮氨酸重复受体BPH14直接结合BISP来激活HPR。Bph14介导免疫的组成性激活对植物的生长和生产力是有害的。Bph14介导的HPR微调是通过BISP和BPH14与选择性自噬货物受体OsNBR1的直接结合实现的，OsNBR1将BISP送到OsATG8降解。因此，自噬作用控制着BISP的水平。在Bph14植物中，当褐飞虱停止取食时，自噬作用通过下调HPR来恢复细胞的平衡。研究人员确定了一个由植物免疫受体感知的昆虫唾液蛋白，并发现了一个三向互动系统，为开发高产、抗虫作物提供了机会。

附：英文原文

Title: A tripartite rheostat controls self-regulated host plant resistance to insects

Author: Guo, Jianping, Wang, Huiying, Guan, Wei, Guo, Qin, Wang, Jing, Yang, Jing, Peng, Yaxin, Shan, Junhan, Gao, Mingyang, Shi, Shaojie, Shangguan, Xinxin, Liu, Bingfang, Jing, Shengli, Zhang, Jing, Xu, Chunxue, Huang, Jin, Rao, Weiwei, Zheng, Xiaohong, Wu, Di, Zhou, Cong, Du, Bo, Chen, Rongzhi, Zhu, Lili, Zhu, Yuxian, Walling, Linda L., Zhang, Qifa, He, Guangcun

Issue&Volume: 2023-06-14

Abstract: Plants deploy receptor-like kinases and nucleotide-binding leucine-rich repeat receptors to confer host plant resistance (HPR) to herbivores1. These gene-for-gene interactions between insects and their hosts have been proposed for more than 50years2. However, the molecular and cellular mechanisms that underlie HPR have been elusive, as the identity and sensing mechanisms of insect avirulence effectors have remained unknown. Here we identify an insect salivary protein perceived by a plant immune receptor. The BPH14-interacting salivary protein (BISP) from the brown planthopper (Nilaparvata lugens Stl) is secreted into rice (Oryza sativa) during feeding. In susceptible plants, BISP targets O.satvia RLCK185 (OsRLCK185; hereafter Os is used to denote O.satvia-related proteins or genes) to suppress basal defences. In resistant plants, the nucleotide-binding leucine-rich repeat receptor BPH14 directly binds BISP to activate HPR. Constitutive activation of Bph14-mediated immunity is detrimental to plant growth and productivity. The fine-tuning of Bph14-mediated HPR is achieved through direct binding of BISP and BPH14 to the selective autophagy cargo receptor OsNBR1, which delivers BISP to OsATG8 for degradation. Autophagy therefore controls BISP levels. In Bph14 plants, autophagy restores cellular homeostasis by downregulating HPR when feeding by brown planthoppers ceases. We identify an insect saliva protein sensed by a plant immune receptor and discover a three-way interaction system that offers opportunities for developing high-yield, insect-resistant crops.

DOI: 10.1038/s41586-023-06197-z

Source: https://www.nature.com/articles/s41586-023-06197-z