中国科学院广州生物医药与健康研究所Guangdun Peng团队近期取得重要工作进展，他们对小鼠大脑发育过程中空间基因表达和染色质可及性进行了同步分析。相关研究成果2023年5月25日在线发表于《自然—方法学》杂志上。

据介绍，大脑是一个复杂的组织，其功能依赖于协调的解剖和分子特征。然而，目前对大脑空间组织的分子注释还不够。

研究人员描述了基于微流体索引的转座酶可及染色质和RNA测序的空间分析（MISAR-seq），这是一种用于染色质可及性和基因表达的空间分辨联合分析的方法。通过将MISAR-seq应用于发育中的小鼠大脑，研究人员探讨了小鼠大脑发育过程中的组织结构和时空调控逻辑。

附：英文原文

Title: Simultaneous profiling of spatial gene expression and chromatin accessibility during mouse brain development

Author: Jiang, Fuqing, Zhou, Xin, Qian, Yingying, Zhu, Miao, Wang, Li, Li, Zhuxia, Shen, Qingmei, Wang, Minhan, Qu, Fangfang, Cui, Guizhong, Chen, Kai, Peng, Guangdun

Issue&Volume: 2023-05-25

Abstract: The brain is a complex tissue whose function relies on coordinated anatomical and molecular features. However, the molecular annotation of the spatial organization of the brain is currently insufficient. Here, we describe microfluidic indexing-based spatial assay for transposase-accessible chromatin and RNA-sequencing (MISAR-seq), a method for spatially resolved joint profiling of chromatin accessibility and gene expression. By applying MISAR-seq to the developing mouse brain, we study tissue organization and spatiotemporal regulatory logics during mouse brain development.

DOI: 10.1038/s41592-023-01884-1

Source: https://www.nature.com/articles/s41592-023-01884-1