通过使用时间序列单细胞RNA测序来描述拟南芥根部不同细胞类型和发育阶段特有的油菜素甾醇反应性基因的表达，研究人员发现伸长皮层是油菜素甾醇触发从增殖到伸长的一个部位，与细胞壁相关基因的表达增加有关。分析显示，HOMEOBOX FROM ARABIDOPSIS THALIANA 7（HAT7）和GT-2-LIKE 1（GTL1）是油菜素甾醇反应性转录因子，调节皮层细胞伸长。
这些结果确立了皮层是油菜素甾醇介导的生长场所，并揭示了调节从增殖到伸长过渡的油菜素甾醇信号网络，它阐明了激素响应的时空方面。
据介绍，油菜素甾醇是植物类固醇激素，通过在空间和时间上不同的基因调控网络来调节各种过程，如细胞分裂和细胞伸长。
附：英文原文
Title: Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root
Author: Trevor M. Nolan, Nemanja Vukainovi, Che-Wei Hsu, Jingyuan Zhang, Isabelle Vanhoutte, Rachel Shahan, Isaiah W. Taylor, Laura Greenstreet, Matthieu Heitz, Anton Afanassiev, Ping Wang, Pablo Szekely, Aiden Brosnan, Yanhai Yin, Geoffrey Schiebinger, Uwe Ohler, Eugenia Russinova, Philip N. Benfey
Issue&Volume: 2023-03-31
Abstract: Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall–related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.
DOI: adf4721
Source: https://www.science.org/doi/10.1126/science.adf4721