武汉大学刘晓庆团队报道了一种用于m6A去甲基酶活性检测的无标记脱氧核酶传感器。相关研究成果发表在2023年12月27日出版的《中国化学》。

研究人员开发了一种检测m6A去甲基化酶活性的无标记脱氧核酶（DNAzyme）生物传感器。当脱甲基酶FTO（脂肪量和肥胖相关蛋白）（ALKBH脱甲基酶家族的一种重要的m6A脱甲基酶）存在时，m6A基团被特异性脱甲基，脱氧核酶的切割活性恢复。这能够使含有G-四链体序列的切割产物与硫黄素T（ThT）结合并产生荧光信号。

该生物传感器具有较高的特异性和灵敏度，反应速度快。研究人员证明了一种用于传感的变构脱氧核酶的新设计。该方法代表了第一个用于FTO测定的无标记核酸生物传感器，为筛选抑制剂提供了可行的途径。

附：英文原文

Title: A Label-free Deoxyribozyme Sensor for m6A Demethylase Activity Detection

Author: Xiuyuan Wang, Shusheng Yu, Yuqiu He, Fuan Wang, Xiaoqing Liu

Issue&Volume: 2023-12-27

Abstract: A label-free deoxyribozyme (DNAzyme) biosensor for m6A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity-associated protein), an important m6A demethylase of ALKBH demethylase family is present, the m6A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label-free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening.

DOI: 10.1002/cjoc.202300633

Source: https://onlinelibrary.wiley.com/doi/10.1002/cjoc.202300633