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原代皮质神经元mRNA定位元件获揭示
作者:小柯机器人 发布时间:2023/1/26 13:04:36

德国柏林医学系统生物学研究所Marina Chekulaeva和以色列魏茨曼科学研究所Igor Ulitsky课题组合作取得一项新成果。经过不懈努力,他们的最新研究鉴定了原代皮质神经元的mRNA定位元件。这一研究成果于2023年1月16日发表在国际学术期刊《自然—神经科学》上。

研究人员建立了一种新的神经元zipcodes识别协议(N-zip),可以识别数百个3'未翻译区域的zipcodes。这种方法将分离神经元主要亚细胞区室(细胞体和神经突)的方法与大规模平行报告蛋白测定相结合。N-zip鉴定到小鼠初级皮质神经元中的let-7结合位点和(AU)n基序为其从头zipcodes。该分析还首次提供了miRNA影响mRNA定位的证据,并提出了检测更多zipcodes的策略。

据介绍,在许多情况下,由于mRNA需要定位到特定区域,细胞采用高度极化的形态并形成不同的亚细胞区室,在那里它们被翻译成具有局部功能的蛋白质。这种mRNA定位由mRNA中特定的顺式调节元件介导,通常称为“zipcodes”。尽管有数百种局部mRNA,但只有zipcodes元件被表征。

附:英文原文

Title: Massively parallel identification of mRNA localization elements in primary cortical neurons

Author: Mendonsa, Samantha, von Kgelgen, Nicolai, Dantsuji, Sayaka, Ron, Maya, Breimann, Laura, Baranovskii, Artem, Ldige, Inga, Kirchner, Marieluise, Fischer, Meret, Zerna, Nadja, Bujanic, Lucija, Mertins, Philipp, Ulitsky, Igor, Chekulaeva, Marina

Issue&Volume: 2023-01-16

Abstract: Cells adopt highly polarized shapes and form distinct subcellular compartments in many cases due to the localization of many mRNAs to specific areas, where they are translated into proteins with local functions. This mRNA localization is mediated by specific cis-regulatory elements in mRNAs, commonly called ‘zipcodes’. Although there are hundreds of localized mRNAs, only a few zipcodes have been characterized. Here we describe a novel neuronal zipcode identification protocol (N-zip) that can identify zipcodes across hundreds of 3′ untranslated regions. This approach combines a method of separating the principal subcellular compartments of neurons—cell bodies and neurites—with a massively parallel reporter assay. N-zip identifies the let-7 binding site and (AU)n motif as de novo zipcodes in mouse primary cortical neurons. Our analysis also provides, to our knowledge, the first demonstration of an miRNA affecting mRNA localization and suggests a strategy for detecting many more zipcodes.

DOI: 10.1038/s41593-022-01243-x

Source: https://www.nature.com/articles/s41593-022-01243-x

期刊信息

Nature Neuroscience:《自然—神经科学》,创刊于1998年。隶属于施普林格·自然出版集团,最新IF:28.771
官方网址:https://www.nature.com/neuro/
投稿链接:https://mts-nn.nature.com/cgi-bin/main.plex