德国马克斯普朗克多学科研究所Stefan Jakobs和Stefan W. Hell合作近期取得重要工作进展，他们最新研究提出了DNA-PAINT MINFLUX纳米镜技术。这一研究成果2022年9月1日在线发表于《自然—方法学》杂志上。

研究人员表明，MINFLUX可以有效地与基于DNA的顺序多重标记（DNA-PAINT）相结合，将MINFLUX纳米技术扩展到多个分子靶点。研究人员以人类细胞中线粒体的三色记录为例证明了他们方法的可行性。

据介绍，最小荧光光子通量（MINFLUX）纳米显微技术，提供了光子效率的荧光团定位，带来了纳米尺度的三维分辨率。然而，通过对单个荧光团分离使用固有的开关过程，最初的MINFLUX实施仅限于两个颜色通道。

附：英文原文

Title: DNA-PAINT MINFLUX nanoscopy

Author: Ostersehlt, Lynn M., Jans, Daniel C., Wittek, Anna, Keller-Findeisen, Jan, Inamdar, Kaushik, Sahl, Steffen J., Hell, Stefan W., Jakobs, Stefan

Issue&Volume: 2022-09-01

Abstract: MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.

DOI: 10.1038/s41592-022-01577-1

Source: https://www.nature.com/articles/s41592-022-01577-1