美国密歇根大学Jianping Fu、Yi Zheng等研究人员合作通过胚状体的单细胞分析揭示人类早期发育的谱系多样化路线图。2022年9月1日出版的《细胞—干细胞》杂志发表了这项成果。

Title: Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development

Author: Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang, Alexander Goedel, Yu Kang, Xufeng Xue, Sajedeh Nasr Esfahani, Yue Liu, Agnes M. Resto Irizarry, Weisheng Wu, Yunxiu Li, Weizhi Ji, Yuyu Niu, Kenneth R. Chien, Tianqing Li, Toshihiro Shioda, Jianping Fu

Issue&Volume: 2022/09/01

Abstract: Despite its clinical and fundamental importance, our understanding of early humandevelopment remains limited. Stem cell-derived, embryo-like structures (or embryoids)allowing studies of early development without using natural embryos can potentiallyhelp fill the knowledge gap of human development. Herein, transcriptome at the single-celllevel of a human embryoid model was profiled at different time points. Molecular mapsof lineage diversifications from the pluripotent human epiblast toward the amnioticectoderm, primitive streak/mesoderm, and primordial germ cells were constructed andcompared with in vivo primate data. The comparative transcriptome analyses reveal a critical role of NODALsignaling in human mesoderm and primordial germ cell specification, which is furtherfunctionally validated. Through comparative transcriptome analyses and validationswith human blastocysts and in vitro cultured cynomolgus embryos, we further proposed stringent criteria for distinguishingbetween human blastocyst trophectoderm and early amniotic ectoderm cells.

DOI: 10.1016/j.stem.2022.08.009

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00345-9