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集成远红荧光探针与微流控平台用于活体红细胞膜动态的超分辨成像
作者:小柯机器人 发布时间:2022/9/30 21:07:04

大连理工大学肖义团队集成远红荧光探针与微流控平台用于活体红细胞膜动态的超分辨成像。相关研究成果于2022年9月19日发表在《德国应用化学》。

依赖于同步超分辨率成像和跟踪,活红细胞(红细胞,RBC)膜呈现出丰富的超微结构和动态细节。然而,由于不同技术的刚性和相互冲突的光物理要求,在单个探针中实现双重功能面临严重挑战。

该文中,研究人员合理研制了具有闪烁能力和一致单分子发射的远红外硼二吡咯烷膜探针,并构建了用于无创捕获和长期成像红细胞的微流控平台。通过将它们结合起来,以高通量的方式在活体人红细胞膜的分子尺度上实现了多维超分辨率重建和单分子追踪。该集成系统定义了一种定量方法,用于在生理和病理条件下分析红细胞膜,提高了精确度,并为未来疾病诊断提供新的视角。

附:英文原文

Title: Integrating a Far-Red Fluorescent Probe with a Microfluidic Platform for Super-Resolution Imaging of Live Erythrocyte Membrane Dynamics

Author: Zhiwei Ye, Wei Yang, Ying Zheng, Shujing Wang, Xiaodong Zhang, Haibo Yu, Shuangshuang Li, Chunxiong Luo, Xiaojun Peng, Yi Xiao

Issue&Volume: 2022-09-19

Abstract: Living erythrocyte (red blood cell, RBC) membranes present rich ultrastructural and dynamic details, which rely on synchronous super-resolution imaging and tracking to unveil. Yet, it poses a serious challenge to achieve the dual functions in a single probe, due to the rigid and conflicting photophysical demands of different techniques. Herein, we rationally developed a far-red boron dipyrromethene membrane probe with blinking capability and consistent single-molecule emission, and constructed a microfluidic platform for noninvasive trapping and long-term imaging of RBCs. By combining them, multi-dimensional super-resolution reconstructions and single-molecule tracking were achieved at the molecular scale on living human RBC membranes in a high-throughput manner. Our integrated system defines a quantitative method for analyzing RBC membranes under physiological and pathological conditions, improving precisions and revealing new perspectives for future disease diagnostics.

DOI: 10.1002/anie.202211540

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202211540

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx