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可逆NIR-IIb荧光氧化还原探针实时追踪肝脏缺血-再灌注损伤
作者:小柯机器人 发布时间:2022/9/22 20:04:10

武汉大学刘志洪团队报道了可逆NIR-IIb荧光氧化还原探针实时追踪肝脏缺血-再灌注损伤。相关研究成果发表在2022年9月19日出版的《德国应用化学》。

肝缺血再灌注损伤(HIRI)是导致肝功能不全的原因,其中活性氧(ROS)是最重要的标志物。HIRI期间实时监测ROS可为早期诊断和及时干预提供重要机会。然而,由于在近红外区域设计可逆响应的荧光探针具有极大的挑战性,目前还没有探针可用于在体内HIRI期间跟踪ROS波动。

该文中,研究人员提出了一种发射波长超过1500 nm的可逆氧化还原探针REPOMs来填补空白,使用掺杂稀土离子的纳米颗粒作为发射体,使用钼基多金属氧酸盐纳米团簇作为ROS识别位点和发射调制器。REPOMs对活性氧和谷胱甘肽之间的重复循环表现出良好的响应,基于此成功地获得了HIRI期间时间分辨的活性氧变化。

该可逆探针可能为今后促进肝病研究提供有力的工具。

附:英文原文

Title: Tracking Hepatic Ischemia-Reperfusion Injury in Real Time with a Reversible NIR-IIb Fluorescent Redox Probe

Author: Dan Song, Chenchen Li, Mengting Zhu, Siyu Chi, Zhihong Liu

Issue&Volume: 2022-09-19

Abstract: Hepatic ischemia-reperfusion injury (HIRI) is responsible for liver dysfunction, which involves reactive oxygen species (ROS) as the most critical marker. Real-time monitoring of ROS during HIRI can provide significant chance for early diagnosis and timely intervention. However, there is no probe available to track ROS fluctuations during HIRI in vivo, as it is extremely challenging to design reversibly responsive fluorescent probe in near-infrared region. Here, a reversible redox probe REPOMs emitting beyond 1500 nm is proposed to fill the blank, using rare earth ions-doped nanoparticles as emitter, and molybdenum-based polyoxometalate nanoclusters as the ROS-recognition site and emission modulator. REPOMs exhibited excellent response towards the repeated cycle between ROS and glutathione, based on which the time-resolved ROS changes during HIRI were successfully obtained. This reversible probe may provide a powerful tool to promote the hepatology research in the future.

DOI: 10.1002/anie.202212721

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202212721

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx