当前位置:科学网首页 > 小柯机器人 >详情
抗天花长效记忆B细胞由脾生态位中动态相互作用和持久的生发中心印记定义
作者:小柯机器人 发布时间:2022/9/22 20:14:52

法国巴黎市立大学Jean-Claude Weill和Claude-Agnès Reynaud共同合作,近期取得重要工作进展,他们研究发现人类抗天花长效记忆B细胞由脾生态位中的动态相互作用和持久的生发中心印记定义。相关研究成果2022年9月20日在线发表于《免疫》杂志。

研究人员分离并分析了40多年前接种疫苗的个体中人类脾脏天花/牛痘蛋白B5特异性MBCs。只有少数克隆在如此长的时间内持续存在,它们表现出有限的克隆内多样性,并具有广泛的基于亲和力选择的迹象。这些长寿的MBCs似乎富含CD21hiCD20hi IgG+脾脏B细胞亚群,显示出边缘区样NOTCH/MYC驱动的特征,但它们没有独特的与长寿相关的转录或代谢谱。最后,在所有分析的样本中,B5特异性、长寿MBCs的端粒都比患者配对的原始B细胞的端粒长。

总体而言,这些结果表明,早期端粒伸长、收缩阶段的亲和力选择和进入特定生态位等有助于确保MBC的功能寿命。

据介绍,记忆B细胞(MBCs)可以存活一生,但它们长期存活的机制仍不清楚。

附:英文原文

Title: Human anti-smallpox long-lived memory B cells are defined by dynamic interactions in the splenic niche and long-lasting germinal center imprinting

Author: Pascal Chappert, Franois Huetz, Marie-Alix Espinasse, Fabrice Chatonnet, Louise Pannetier, Lucie Da Silva, Clara Goetz, Jérome Mégret, Aurélien Sokal, Etienne Crickx, Ivan Nemazanyy, Vincent Jung, Chiara Guerrera, Sébastien Storck, Matthieu Mahévas, Antonio Cosma, Patrick Revy, Thierry Fest, Claude-Agnès Reynaud, Jean-Claude Weill

Issue&Volume: 2022-09-20

Abstract: Memory B cells (MBCs) can persist for a lifetime, but the mechanisms that allow their long-term survival remain poorly understood. Here, we isolated and analyzed human splenic smallpox/vaccinia protein B5-specific MBCs in individuals who were vaccinated more than 40 years ago. Only a handful of clones persisted over such an extended period, and they displayed limited intra-clonal diversity with signs of extensive affinity-based selection. These long-lived MBCs appeared enriched in a CD21hiCD20hi IgG+ splenic B cell subset displaying a marginal-zone-like NOTCH/MYC-driven signature, but they did not harbor a unique longevity-associated transcriptional or metabolic profile. Finally, the telomeres of B5-specific, long-lived MBCs were longer than those in patient-paired naive B cells in all the samples analyzed. Overall, these results imply that separate mechanisms such as early telomere elongation, affinity selection during the contraction phase, and access to a specific niche contribute to ensuring the functional longevity of MBCs.

DOI: 10.1016/j.immuni.2022.08.019

Source: https://www.cell.com/immunity/fulltext/S1074-7613(22)00413-7

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx