山西大学杨恒权报道了连续流动催化中Pickering乳化液液滴内酶和辅助因子的共区化。相关研究成果发表在2022年9月16日出版的国际学术期刊《德国应用化学》。

由于难以确保固定化酶和辅因子的自由可及性，以适合于连续流动催化的方式对酶和辅因素进行共固定仍然是一个巨大的挑战。

该文中，研究人员提出了一种基于Pickering乳状液滴内酶和辅因子共分区的连续流催化系统，它可以实现液滴内的酶和辅因素共固定化以及辅因子再生。如酶催化酮对映选择性还原和对映选择性转氨作用所示，与传统两相反应相比，该体系具有长期稳定性（300-400 h）、显著的总周转数（TTN，59204 mol mol^-1）和酶催化效率（CE e）的几倍提高。

该方法以及对共区化效应的深入了解，将为大幅度推进连续流生物催化的实际应用水平提供巨大的机会。

附：英文原文

Title: Co-compartmentalization of Enzymes and Cofactors within Pickering Emulsion Droplets for Continuous Flow Catalysis

Author: Wei Wei, Rammile Ettelaie, Xiaoming Zhang, Min Fan, Yue Dong, Zebiao Li, Hengquan Yang

Issue&Volume: 2022-09-16

Abstract: Co-immobilization of enzymes and cofactors in a manner suitable for use in continuous flow catalysis remains a great challenge because of the difficulty in ensuring the free accessibility of immobilized enzymes and cofactors. Herein, we present a continuous flow catalysis system based on co-compartmentalization of enzymes and cofactors within Pickering emulsion droplets, which can realize co-immobilized enzymes and cofactors and regeneration of cofactors within the droplets. As exemplified by enzyme-catalyzed ketone enantioselective reduction and enantioselective transamination, our systems exhibit long-term stability (300-400 h), outstanding total turnover number (TTN, 59204 mol mol -1  ) and several-fold enhancement in the enzyme catalytic efficiency (CE e  ) in comparison to conventional biphasic reactions. Our method along with revealing insight into the co-compartmentalization effects will provide a vast opportunity for significantly advancing continuous flow biocatalysis towards the level of practical applications.

DOI: 10.1002/anie.202211912

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202211912