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用于远平衡化学网络可扩展工程的标准化可激发元件
作者:小柯机器人 发布时间:2022/8/7 18:06:32

美国约翰斯·霍普金斯大学Schulman, Rebecca团队研制用于远平衡化学网络可扩展工程的标准化可激发元件。该项研究成果发表在2022年8月4日出版的《自然—化学》上。

设计远离平衡的,根据环境信号脉冲或切换状态的合成化学网络,可以精确调节化学合成或自组装的动力学。目前,必须对此类网络进行广泛调整,以补偿网络各种化学成分之间的不同活动和意外反应。具有标准化性能的模块化元件可用于快速构建具有设计功能的网络。

该文中,研究人员开发了标准化的易激调控元件,称为小基因,并使用它们构建复杂的体外转录网络。研究人员开发了用于识别性能一致,串扰最小的超过15个可互换的小基因元件。这些元件可以结合起来设计前馈和反馈模块,其动力学与简单动力学模型预测的动力学匹配。然后,模块可以合理地集成和组织成网络,产生可调谐的时间脉冲,并充当多状态可切换存储器。

标准化的小基因元件和识别更多元素的工作流程应使工程变得复杂,远离平衡化学动力学常规。与活细胞中的遗传调节网络类似的远离平衡动力学的合成化学网络可以精确地调节化学合成或自组装的动力学。现在,已开发出标准化的可激化学调节元件,称为小基因,可预测自底向上构建具有设计的时间和多稳态行为的体外网络。

附:英文原文

Title: Standardized excitable elements for scalable engineering of far-from-equilibrium chemical networks

Author: Schaffter, Samuel W., Chen, Kuan-Lin, OBrien, Jackson, Noble, Madeline, Murugan, Arvind, Schulman, Rebecca

Issue&Volume: 2022-08-04

Abstract: Engineered far-from-equilibrium synthetic chemical networks that pulse or switch states in response to environmental signals could precisely regulate the kinetics of chemical synthesis or self-assembly. Currently, such networks must be extensively tuned to compensate for the different activities of and unintended reactions between a network’s various chemical components. Modular elements with standardized performance could be used to rapidly construct networks with designed functions. Here we develop standardized excitable chemical regulatory elements, termed genelets, and use them to construct complex in vitro transcriptional networks. We develop a protocol for identifying >15 interchangeable genelet elements with uniform performance and minimal crosstalk. These elements can be combined to engineer feedforward and feedback modules whose dynamics match those predicted by a simple kinetic model. Modules can then be rationally integrated and organized into networks that produce tunable temporal pulses and act as multistate switchable memories. Standardized genelet elements, and the workflow to identify more, should make engineering complex far-from-equilibrium chemical dynamics routine. Synthetic chemical networks with far-from-equilibrium dynamics akin to genetic regulatory networks in living cells could precisely regulate the kinetics of chemical synthesis or self-assembly. Now standardized excitable chemical regulatory elements, termed genelets, that enable predictive bottom-up construction of in vitro networks with designed temporal and multistable behaviour have been developed.

DOI: 10.1038/s41557-022-01001-3

Source: https://www.nature.com/articles/s41557-022-01001-3

期刊信息

Nature Chemistry:《自然—化学》,创刊于2009年。隶属于施普林格·自然出版集团,最新IF:21.687
官方网址:https://www.nature.com/nchem/
投稿链接:https://mts-nchem.nature.com/cgi-bin/main.plex