西澳大利亚大学Ryan Lister研究小组在植物中开发出用于稳定细胞重编程的合成记忆回路。相关论文于2022年7月4日在线发表在《自然—生物技术》杂志上。

Author: Lloyd, James P. B., Ly, Florence, Gong, Patrick, Pflueger, Jahnvi, Swain, Tessa, Pflueger, Christian, Fourie, Elliott, Khan, Muhammad Adil, Kidd, Brendan N., Lister, Ryan

Issue&Volume: 2022-07-04

Abstract: Plant biotechnology predominantly relies on a restricted set of genetic parts with limited capability to customize spatiotemporal and conditional expression patterns. Synthetic gene circuits have the potential to integrate multiple customizable input signals through a processing unit constructed from biological parts to produce a predictable and programmable output. Here we present a suite of functional recombinase-based gene circuits for use in plants. We first established a range of key gene circuit components compatible with plant cell functionality. We then used these to develop a range of operational logic gates using the identify function (activation) and negation function (repression) in Arabidopsis protoplasts and in vivo, demonstrating their utility for programmable manipulation of transcriptional activity in a complex multicellular organism. Specifically, using recombinases and plant control elements, we activated transgenes in YES, OR and AND gates and repressed them in NOT, NOR and NAND gates; we also implemented the A NIMPLY B gate that combines activation and repression. Through use of genetic recombination, these circuits create stable long-term changes in expression and recording of past stimuli. This highly compact programmable gene circuit platform provides new capabilities for engineering sophisticated transcriptional programs and previously unrealized traits into plants. Transcriptional activity in plants is controlled with a programmable gene circuit.

DOI: 10.1038/s41587-022-01383-2

Source: https://www.nature.com/articles/s41587-022-01383-2