德国马克斯·普朗克医学研究所Hell, Stefan W.团队开发了，用于活细胞纳米观察的无笼基团光激活荧光团的通用设计策略。相关研究成果发表在2022年7月21日出版的《自然—化学》。

荧光团在非荧光和荧光状态之间的受控切换是每种超分辨率荧光显微镜（纳米镜）技术的核心，探索全新的切换机制对于提高现有和新兴超分辨率方法的性能仍然至关重要。光活化染料对许多这类技术都有重大改进，但通常依赖于光不稳定的保护基团，限制了其应用。

该文中，研究人员描述了一种将3,6-二氨基花色酮转化为无笼基团的光激活荧光团的通用方法。这些光激活的黄原酮（PaX）在光照射下快速、清洁地组装成高荧光、光稳定性和化学稳定性的吡咯啉染料。该策略可扩展到碳和硅桥联的蒽酮类似物，产生一系列跨越大部分可见光谱的光激活标签。结果证明了PaX染料在常规显微镜的固定和活细胞标记中的多功能性和实用性，以及在STED、PALM和MINFLUX的坐标随机和确定性纳米观察中的多功能性和实用性。

附：英文原文

Title: A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy

Author: Lincoln, Richard, Bossi, Mariano L., Remmel, Michael, DEste, Elisa, Butkevich, Alexey N., Hell, Stefan W.

Issue&Volume: 2022-07-21

Abstract: The controlled switching of fluorophores between non-fluorescent and fluorescent states is central to every super-resolution fluorescence microscopy (nanoscopy) technique, and the exploration of radically new switching mechanisms remains critical to boosting the performance of established, as well as emerging super-resolution methods. Photoactivatable dyes offer substantial improvements to many of these techniques, but often rely on photolabile protecting groups that limit their applications. Here we describe a general method to transform 3,6-diaminoxanthones into caging-group-free photoactivatable fluorophores. These photoactivatable xanthones (PaX) assemble rapidly and cleanly into highly fluorescent, photo- and chemically stable pyronine dyes upon irradiation with light. The strategy is extendable to carbon- and silicon-bridged xanthone analogues, yielding a family of photoactivatable labels spanning much of the visible spectrum. Our results demonstrate the versatility and utility of PaX dyes in fixed and live-cell labelling for conventional microscopy, as well as the coordinate-stochastic and deterministic nanoscopies STED, PALM and MINFLUX.

DOI: 10.1038/s41557-022-00995-0

Source: https://www.nature.com/articles/s41557-022-00995-0