奥地利科学院Nicolas C. Rivron研究组发现，外胚层诱导剂可在体外捕获小鼠滋养层干细胞。相关论文发表在2022年7月7日出版的《细胞—干细胞》杂志上。

研究人员定义了一套由外胚层分泌的最佳分子（诱导剂），这能够在体外捕获稳定的、高度自我更新的小鼠滋养层干细胞（TESC），类似于囊胚阶段。当暴露在不理想的诱导剂下时，这些干细胞会波动形成可相互转换的亚群，自我更新能力降低，分化能力增强，类似于植入前的细胞，被称为滋养层（TR）干细胞（TSC）。

TESC有更强的能力形成blastoid，由于诱导剂不仅维持局部TR的增殖和自我更新，而且维持WNT6/7B的分泌，刺激子宫蜕膜化，所以在子宫内更有效地植入。总的来说，外胚层维持着持续的生长和相邻TR细胞的蜕膜化潜力，同时，如已知的那样，囊腔强加的距离使TR细胞分化为子宫粘附，从而形成了植入的基本功能。

据了解，胚胎指示细胞状态的分配，从而在空间上调节功能。在囊胚中，TR细胞的模式化确保了成功植入和胎盘发育。

附：英文原文

Title: Epiblast inducers capture mouse trophectoderm stem cells in vitro and pattern blastoids for implantation in utero

Author: Jinwoo Seong, Javier Frias-Aldeguer, Viktoria Holzmann, Harunobu Kagawa, Giovanni Sestini, Heidar Heidari Khoei, Yvonne Scholte Op Reimer, Maarten Kip, Saurabh J. Pradhan, Lucas Verwegen, Judith Vivié, Linfeng Li, Anna Alemany, Jeroen Korving, Frank Darmis, Alexander van Oudenaarden, Derk ten Berge, Niels Geijsen, Nicolas C. Rivron

Issue&Volume: 2022/07/07

Abstract: The embryo instructs the allocation of cell states to spatially regulate functions.In the blastocyst, patterning of trophoblast (TR) cells ensures successful implantationand placental development. Here, we defined an optimal set of molecules secreted bythe epiblast (inducers) that captures in vitro stable, highly self-renewing mouse trophectoderm stem cells (TESCs) resembling theblastocyst stage. When exposed to suboptimal inducers, these stem cells fluctuateto form interconvertible subpopulations with reduced self-renewal and facilitateddifferentiation, resembling peri-implantation cells, known as TR stem cells (TSCs).TESCs have enhanced capacity to form blastoids that implant more efficiently in utero due to inducers maintaining not only local TR proliferation and self-renewal, butalso WNT6/7B secretion that stimulates uterine decidualization. Overall, the epiblastmaintains sustained growth and decidualization potential of abutting TR cells, while,as known, distancing imposed by the blastocyst cavity differentiates TR cells foruterus adhesion, thus patterning the essential functions of implantation.

DOI: 10.1016/j.stem.2022.06.002

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00251-X