瑞典卡洛琳卡学院Rickard Sandberg研究小组开发出Smart-seq3xpress，实现完整转录本的单细胞RNA测序。相关论文于2022年5月30日在线发表在《自然—生物技术》杂志上。

研究人员报道了Smart-seq3xpress，它使Smart-seq3方案小型化和精简，大大减少了试剂的使用并提高了细胞的通量。对外周血单核细胞的Smart-seq3xpress分析产生了一个完整的颗粒状图谱，包括常见和罕见的细胞类型。与基于液滴的单细胞RNA测序对RNA末端进行测序相比，额外的全转录覆盖揭示了与细胞类型相关的亚型变化。

据介绍，目前具有高细胞通量的单细胞RNA测序方法牺牲了全转录覆盖率，而且往往牺牲了灵敏度。

附：英文原文

Title: Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress

Author: Hagemann-Jensen, Michael, Ziegenhain, Christoph, Sandberg, Rickard

Issue&Volume: 2022-05-30

Abstract: Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughput. Smart-seq3xpress analysis of peripheral blood mononuclear cells resulted in a granular atlas complete with common and rare cell types. Compared with droplet-based single-cell RNA sequencing that sequences RNA ends, the additional full-transcript coverage revealed cell-type-associated isoform variation. Smart-seq3xpress enables single-cell RNA sequencing from full transcripts at high throughput.

DOI: 10.1038/s41587-022-01311-4

Source: https://www.nature.com/articles/s41587-022-01311-4