在这里，研究人员鉴定了一个数量性状基因座，Thermo-tolerance 3 (TT3)，它由两个基因TT3.1和TT3.2组成，它们相互作用以增强水稻耐热性并减少由热胁迫引起的谷物产量损失。 在热胁迫下，质膜定位的E3连接酶TT3.1易位到核内体，TT3.1泛素化叶绿体前体蛋白TT3.2进行液泡降解，这意味着TT3.1可能作为一种潜在的热传感器。 叶绿体中积累较少的成熟TT3.2蛋白对于保护类囊体免受热胁迫至关重要。
他们的研究结果不仅揭示了一个基因座上的TT3.1-TT3.2遗传模块，该基因模块将热信号从质膜传递到叶绿体，而且还为培育高耐热作物提供了策略。
据介绍，质膜如何感知外界的热胁迫信号，并与叶绿体沟通，以协调热耐受性仍然是一个重要的问题。
附：英文原文
Title: A genetic module at one locus in rice protects chloroplasts to enhance thermotolerance
Author: Hai Zhang, Ji-Fu Zhou, Yi Kan, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Tao Guo, You-Huang Xiang, Yi-Bing Yang, Ya-Chao Li, Huai-Yu Zhao, Hong-Xiao Yu, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Xiao-Rui Mu, Ying-Jie Cao, Jia-Jun Yu, Youshun Lin, Hong-Xuan Lin
Issue&Volume: 2022-06-17
Abstract: How the plasma membrane senses external heat-stress signals to communicate with chloroplasts to orchestrate thermotolerance remains elusive. We identified a quantitative trait locus, Thermo-tolerance 3 (TT3), consisting of two genes, TT3.1 and TT3.2, that interact together to enhance rice thermotolerance and reduce grain-yield losses caused by heat stress. Upon heat stress, plasma membrane–localized E3 ligase TT3.1 translocates to the endosomes, on which TT3.1 ubiquitinates chloroplast precursor protein TT3.2 for vacuolar degradation, implying that TT3.1 might serve as a potential thermosensor. Lesser accumulated, mature TT3.2 proteins in chloroplasts are essential for protecting thylakoids from heat stress. Our findings not only reveal a TT3.1-TT3.2 genetic module at one locus that transduces heat signals from plasma membrane to chloroplasts but also provide the strategy for breeding highly thermotolerant crops.
DOI: abo5721
Source: https://www.science.org/doi/10.1126/science.abo5721