英国惠康基因组研究园区Sarah A. Teichmann、Muzlifah Haniffa和Menna R. Clatworthy研究小组合作绘制了发育过程中人体免疫系统跨器官图谱。相关论文于2022年5月12日发表在《科学》杂志上。

研究人员将单细胞RNA测序、抗原受体测序和空间转录组学分析相结合分析了九种产前组织，以重建发育过程中人类免疫系统的构成。研究揭示了骨髓和淋巴细胞亚群较晚获得免疫效应功能以及单核细胞和T细胞的成熟早于外周组织发育。

此外，研究发现了原代造血器官以外的全系统血液和免疫细胞发育，这代表了人类产前B1细胞，并阐明了非常规T细胞的起源。该图谱集提供了宝贵的数据资源和生物学见解，将促进细胞工程、再生医学的发展以及对疾病的理解。

研究人员表示，单细胞基因组学研究已经破解了几种人类产前器官的免疫细胞组成，但在发育中免疫系统跨组织分布网络揭示方面受到限制。

附：英文原文

Title: Mapping the developing human immune system across organs

Author: Chenqu Suo, Emma Dann, Issac Goh, Laura Jardine, Vitalii Kleshchevnikov, Jong-Eun Park, Rachel A. Botting, Emily Stephenson, Justin Engelbert, Zewen Kelvin Tuong, Krzysztof Polanski, Nadav Yayon, Chuan Xu, Ondrej Suchanek, Rasa Elmentaite, Cecilia Domínguez Conde, Peng He, Sophie Pritchard, Mohi Miah, Corina Moldovan, Alexander S. Steemers, Pavel Mazin, Martin Prete, Dave Horsfall, John C. Marioni, Menna R. Clatworthy, Muzlifah Haniffa, Sarah A. Teichmann

Issue&Volume: 2022-05-12

Abstract: Single-cell genomics studies have decoded the immune-cell composition of several human prenatal organs but were limited in understanding the developing immune system as a distributed network across tissues. We profiled nine prenatal tissues combining single-cell RNA sequencing, antigen-receptor sequencing, and spatial transcriptomics to reconstruct the developing human immune system. This revealed the late acquisition of immune effector functions by myeloid and lymphoid cell subsets and the maturation of monocytes and T cells prior to peripheral tissue seeding. Moreover, we uncovered system-wide blood and immune cell development beyond primary hematopoietic organs, characterized human prenatal B1 cells, and shed light on the origin of unconventional T cells. Our atlas provides both valuable data resources and biological insights that will facilitate cell engineering, regenerative medicine, and disease understanding.

DOI: abo0510

Source: https://www.science.org/doi/10.1126/science.abo0510