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Trans-Seq绘制出一个选择性的哺乳动物视网膜突触
作者:小柯机器人 发布时间:2022/5/11 14:02:27

近日,美国加州大学旧金山分校Xin Duan研究组利用Trans-Seq绘制出一个选择性的哺乳动物视网膜突触。2022年5月6日出版的《自然—神经科学》杂志发表了这项成果。

研究人员开发了一种综合方法,称为Trans-Seq,可用于绘制视网膜神经节细胞(RGC)到上丘脑(SC)回路。Trans-Seq结合了荧光前向跨突触示踪剂,包括编码优化的小麦胚芽凝集素与mCherry融合,以及单细胞RNA测序。研究人员用Trans-Seq对由遗传定义的RGC类型支配的SC神经元类型进行分类,并预测了从αRGC到Nephronectin阳性宽场神经元(NPWF)的神经元配对。研究人员用基因标记、电生理学和逆行追踪来验证这种联系。然后,研究人员利用Trans-Seq的转录组数据确定了Nephronectin是通过与Integrin α8β1结合,从αRGC到NPWF选择性突触选择的决定因素。Trans-Seq方法可广泛用于从基因定义的突触前神经元发现突触后回路。
 
据悉,小鼠视觉系统是了解哺乳动物回路连接的一个易得模型。尽管在视网膜回路方面有丰富的知识,但从不同的RGC类型到不同的脑神经元类型的长程连接图仍然是未知的。
 
附:英文原文
 
Title: Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin

Author: Tsai, Nicole Y., Wang, Fei, Toma, Kenichi, Yin, Chen, Takatoh, Jun, Pai, Emily L., Wu, Kongyan, Matcham, Angela C., Yin, Luping, Dang, Eric J., Marciano, Denise K., Rubenstein, John L., Wang, Fan, Ullian, Erik M., Duan, Xin

Issue&Volume: 2022-05-06

Abstract: The mouse visual system serves as an accessible model to understand mammalian circuit wiring. Despite rich knowledge in retinal circuits, the long-range connectivity map from distinct retinal ganglion cell (RGC) types to diverse brain neuron types remains unknown. In this study, we developed an integrated approach, called Trans-Seq, to map RGCs to superior collicular (SC) circuits. Trans-Seq combines a fluorescent anterograde trans-synaptic tracer, consisting of codon-optimized wheat germ agglutinin fused to mCherry, with single-cell RNA sequencing. We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from αRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used transcriptomic data from Trans-Seq to identify Nephronectin as a determinant for selective synaptic choice from αRGC to NPWFs via binding to Integrin α8β1. The Trans-Seq approach can be broadly applied for post-synaptic circuit discovery from genetically defined pre-synaptic neurons.

DOI: 10.1038/s41593-022-01068-8

Source: https://www.nature.com/articles/s41593-022-01068-8

期刊信息

Nature Neuroscience:《自然—神经科学》,创刊于1998年。隶属于施普林格·自然出版集团,最新IF:21.126
官方网址:https://www.nature.com/neuro/
投稿链接:https://mts-nn.nature.com/cgi-bin/main.plex