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研究揭示淋巴结基质前体的起源和多样性
作者:小柯机器人 发布时间:2022/4/4 12:50:34

意大利圣拉斐尔科学研究所Andrea Brendolan课题组揭示淋巴结基质前体的起源和多样性 。相关论文于2022年3月30日在线发表在《免疫》杂志上。

研究人员使用系谱追踪方法和单细胞转录组分析来确定了淋巴结(LN)基质细胞和内皮祖细胞的起源、转录概况和组成。结果显示,所有主要的基质细胞亚群和很大一部分血液内皮细胞起源于胚胎时期的侧板中胚层(LPM)的Hoxb6+祖细胞,而淋巴内皮细胞产生于旁轴中胚层(PXM)的Pax3+祖细胞。单细胞RNA测序显示存在不同的Cd34+和Cxcl13+基质细胞亚群,并显示胚胎LN含有增殖的祖细胞,可能代表终末分化细胞的扩增群体。

总之,这项工作确定了LN基质和内皮细胞的最早胚胎来源,并证明了基质的多样性在LN发育过程中已经开始。

据介绍,LN基质细胞在LN发育和支持适应性免疫反应中起着关键作用。然而,它们的起源、分化途径和转录程序仍然是难以捉摸的。

附:英文原文

Title: Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors

Author: Elisa Lenti, Luca Genovese, Silvia Bianchessi, Aurora Maurizio, Simona Baghai Sain, Alessia di Lillo, Greta Mattavelli, Itamar Harel, Francesca Bernassola, Thomas Hehlgans, Klaus Pfeffer, Mariacristina Crosti, Sergio Abrignani, Sylvia M. Evans, Giovanni Sitia, Nuno Guimares-Camboa, Vincenzo Russo, Serge A. van de Pavert, Jose Manuel Garcia-Manteiga, Andrea Brendolan

Issue&Volume: 2022-03-30

Abstract: Lymph node (LN) stromal cells play a crucial role in LN development and in supportingadaptive immune responses. However, their origin, differentiation pathways, and transcriptionalprograms are still elusive. Here, we used lineage-tracing approaches and single-celltranscriptome analyses to determine origin, transcriptional profile, and compositionof LN stromal and endothelial progenitors. Our results showed that all major stromalcell subsets and a large proportion of blood endothelial cells originate from embryonicHoxb6+ progenitors of the lateral plate mesoderm (LPM), whereas lymphatic endothelial cellsarise from Pax3+ progenitors of the paraxial mesoderm (PXM). Single-cell RNA sequencing revealed theexistence of different Cd34+ and Cxcl13+ stromal cell subsets and showed that embryonic LNs contain proliferating progenitorspossibly representing the amplifying populations for terminally differentiated cells.Taken together, our work identifies the earliest embryonic sources of LN stromal andendothelial cells and demonstrates that stromal diversity begins already during LNdevelopment.

DOI: 10.1016/j.immuni.2022.03.002

Source: https://www.cell.com/immunity/fulltext/S1074-7613(22)00125-X

 

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx