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蛋白质和核酸联合成像揭示病毒依赖的B细胞和巨噬细胞对组织微环境的免疫抑制作用
作者:小柯机器人 发布时间:2022/4/24 14:14:08

美国斯坦福大学Garry P. Nolan等研究人员合作利用蛋白质和核酸联合成像,揭示病毒依赖的B细胞和巨噬细胞对组织微环境的免疫抑制作用。2022年4月20日,国际知名学术期刊《免疫》在线发表了这一成果。

研究人员开发了蛋白质和核酸原位成像(PANINI),能够同时量化这些组织区间内的DNA、RNA和蛋白质水平。通过将PANINI与多路离子束成像(MIBI)相结合,研究人员同时测量了来自健康或感染了模拟免疫缺陷病毒(SIV)的非人类灵长类动物的存档淋巴组织中的30多个参数。PANINI使细胞表型、功能标志物和感染引起的病毒事件的空间剖析成为可能。SIV感染诱导了淋巴B细胞中IL-10的表达,这与局部巨噬细胞M2极化相关。这突出了一个潜在的病毒机制,即为病毒的产生调节免疫抑制的组织环境。这个空间多模态框架可以扩展到破译其他传染病和肿瘤生物学的组织反应。

据悉,要了解HIV组织的持续存在机制,就必须有能力将感染细胞所在的组织微环境可视化;然而,技术障碍限制了人们剖析这些HIV储库的细胞成分。

附:英文原文

Title: Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments

Author: Sizun Jiang, Chi Ngai Chan, Xavier Rovira-Clavé, Han Chen, Yunhao Bai, Bokai Zhu, Erin McCaffrey, Noah F. Greenwald, Candace Liu, Graham L. Barlow, Jason L. Weirather, John Paul Oliveria, Tsuguhisa Nakayama, Ivan T. Lee, Matthias S. Matter, Anne E. Carlisle, Darci Philips, Gustavo Vazquez, Nilanjan Mukherjee, Kathleen Busman-Sahay, Michael Nekorchuk, Margaret Terry, Skyler Younger, Marc Bosse, Janos Demeter, Scott J. Rodig, Alexandar Tzankov, Yury Goltsev, David Robert McIlwain, Michael Angelo, Jacob D. Estes, Garry P. Nolan

Issue&Volume: 2022-04-20

Abstract: Understanding the mechanisms of HIV tissue persistence necessitates the ability to visualize tissue microenvironments where infected cells reside; however, technological barriers limit our ability to dissect the cellular components of these HIV reservoirs. Here, we developed protein and nucleic acid in situ imaging (PANINI) to simultaneously quantify DNA, RNA, and protein levels within these tissue compartments. By coupling PANINI with multiplexed ion beam imaging (MIBI), we measured over 30 parameters simultaneously across archival lymphoid tissues from healthy or simian immunodeficiency virus (SIV)-infected nonhuman primates. PANINI enabled the spatial dissection of cellular phenotypes, functional markers, and viral events resulting from infection. SIV infection induced IL-10 expression in lymphoid B cells, which correlated with local macrophage M2 polarization. This highlights a potential viral mechanism for conditioning an immunosuppressive tissue environment for virion production. The spatial multimodal framework here can be extended to decipher tissue responses in other infectious diseases and tumor biology.

DOI: 10.1016/j.immuni.2022.03.020

Source: https://www.cell.com/immunity/fulltext/S1074-7613(22)00143-1

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx