美国国立卫生研究院Martin Kampmann研究团队建立了用于大规模合作研究的参考人类诱导多能干细胞(iPSC)。相关论文发表在2022年12月1日出版的《细胞—干细胞》杂志上。

他们亚克隆了候选的人类iPSC细胞系，并通过全基因组测序、基于CRISPR-Cas9基因编辑的基因组稳定性，以及包括常用细胞类型分化在内的表型特性对其遗传特性进行了深入分析。这些研究确定KOLF2.1J是一个全能的性能良好的iPSC细胞系。然后，他们将KOLF2.1J与世界各地的团队分享，他们通过不同的分化协议和功能测试，将其与自己首选的iPSC细胞系进行直接比较，测试其性能。基于这些发现，他们已经使KOLF2.1J及其基因编辑的衍生克隆易于获取，以促进干细胞领域大规模合作科学所需的标准化。

研究人员表示，iPSC细胞系是研究发育和疾病的有力工具，但系间显著的表型差异使复制关键发现和跨研究小组整合数据具有挑战性。

附：英文原文

Title: A reference human induced pluripotent stem cell line for large-scale collaborative studies

Author: Caroline B. Pantazis, Andrian Yang, Erika Lara, Justin A. McDonough, Cornelis Blauwendraat, Lirong Peng, Hideyuki Oguro, Jitendra Kanaujiya, Jizhong Zou, David Sebesta, Gretchen Pratt, Erin Cross, Jeffrey Blockwick, Philip Buxton, Lauren Kinner-Bibeau, Constance Medura, Christopher Tompkins, Stephen Hughes, Marianita Santiana, Faraz Faghri, Mike A. Nalls, Daniel Vitale, Shannon Ballard, Yue A. Qi, Daniel M. Ramos, Kailyn M. Anderson, Julia Stadler, Priyanka Narayan, Jason Papademetriou, Luke Reilly, Matthew P. Nelson, Sanya Aggarwal, Leah U. Rosen, Peter Kirwan, Venkat Pisupati, Steven L. Coon, Sonja W. Scholz, Theresa Priebe, Miriam ttl, Jian Dong, Marieke Meijer, Lara J.M. Janssen, Vanessa S. Lourenco, Rik van der Kant, Dennis Crusius, Dominik Paquet, Ana-Caroline Raulin, Guojun Bu, Aaron Held, Brian J. Wainger, Rebecca M.C. Gabriele, Jackie M. Casey, Selina Wray, Dad Abu-Bonsrah, Clare L. Parish, Melinda S. Beccari, Don W. Cleveland, Emmy Li, Indigo V.L. Rose, Martin Kampmann

Issue&Volume: 2022/12/01

Abstract: Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.

DOI: 10.1016/j.stem.2022.11.004

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00451-9