北京大学陈兴团队报道了细菌间化学交流引发的新生蛋白质组学。相关研究成果于2022年11月25日发表在《德国应用化学》。

用可点击的非经典氨基酸进行代谢标记使新生蛋白质组分析成为可能，并且可以以细胞类型特定的方式进行。然而，以细胞间通信依赖方式的新生蛋白质组学仍然具有挑战性。

该文中，研究人员开发了蛋白质表达的通信激活谱（CAPPEX），它集成了LuxI/LuxR群体传感电路与细胞类型特异性新生蛋白质组学方法，以在接收到来自另一种报告细菌的化学信号时，对特定细菌中新合成的蛋白质进行选择性点击标记。CAPPEX揭示，大肠杆菌与沙门氏菌竞争色氨酸作为吲哚的前体，由此产生的吲哚抑制了沙门氏菌中毒力因子的表达。这种色氨酸吲哚轴可以减弱沙门氏菌在宿主细胞和活小鼠中的入侵。CAPPEX策略广泛适用于研究各种细菌间传播过程。

附：英文原文

Title: Interbacterial Chemical Communication-Triggered Nascent Proteomics

Author: Weibing Liu, Qi Tang, Liying Meng, Shufan Hu, Deen Sun, Shan Li, Peng Dai, Xing Chen

Issue&Volume: 2022-11-25

Abstract: Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell-type-specific manner. However, nascent proteomics in an intercellular communication-dependent manner remains challenging. Here we develop communication-activated profiling of protein expression (CAPPEX), which integrates the LuxI/LuxR quorum sensing circuit with the cell-type-specific nascent proteomics method to enable selective click-labeling of newly synthesized proteins in a specific bacterium upon receiving chemical signals from another reporter bacterium. CAPPEX reveals that E. coli competes with Salmonella for tryptophan as the precursor for indole, and the resulting indole suppressed the expression of virulence factors in Salmonella. This tryptophan-indole axis confers attenuation of Salmonella invasion in host cells and living mice. The CAPPEX strategy should be widely applicable for investigating various interbacterial communication processes.

DOI: 10.1002/anie.202214010

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202214010