清华大学陈永湘团队报道了用于金属蛋白设计的遗传编码膦配体。相关研究成果于2022年11月23日发表在《美国化学会杂志》。

膦配体由于其独特的电子和空间性质，是交叉偶联反应中最重要的一类配体。然而，金属蛋白通常依赖于氮、硫或氧配体。

该文中，研究人员报道了含有生物相容的硼烷保护磷化氢的P3BF基因掺入蛋白。这一步骤之后是一个简单的一锅策略，以在水和有氧条件下进行脱氮和钯配位。基因编码的磷化氢配体P3BF能显著提高设计功能性金属蛋白的能力。

附：英文原文

Title: Genetically Encoded Phosphine Ligand for Metalloprotein Design

Author: Hua-Zhen Duan, Cheng Hu, Yue-Lin Li, Shi-Hao Wang, Yan Xia, Xiaohong Liu, Jiangyun Wang, Yong-Xiang Chen

Issue&Volume: November 23, 2022

Abstract: Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.

DOI: 10.1021/jacs.2c09683

Source: https://pubs.acs.org/doi/10.1021/jacs.2c09683