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MINSTED纳米显微技术进入angstrom定位范围
作者:小柯机器人 发布时间:2022/11/9 14:54:12

德国马克斯普朗克多学科科学研究所Stefan W. Hell团队近期取得重要工作进展,他们研究开发了MINSTED纳米显微技术,实现了Ångström范围的定位精度。相关论文2022年11月7日在线发表于《自然—生物技术》杂志上。

研究人员报道了荧光团的全光、室温定位,精度在Ångström范围内。他们基于MINSTED纳米显微学技术的概念,通过用受激发射耗尽(STED)环形束的低强度中心区域包围荧光团,同时不断增加绝对环形功率,从而提高了精度。通过蓝移STED光束并通过开/关开关分离荧光团,与DNA链结合的单个荧光团用σ=4.7Å,相当于荧光团大小的一小部分,仅检测到2000个光子。

研究人员通过成像核孔复合物和瞬时DNA杂交标记的哺乳动物细胞中核层蛋白的分布,来证明MINSTED荧光纳米显微技术具有个位数的纳米分辨率。实验产生了定位精度σ=2.3Å,估计有10000个探测到的光子,研究人员预计MINSTED将在细胞大分子复合物的研究中开辟新的应用领域。

据介绍,超分辨技术已经实现了纳米级的定位精度。

附:英文原文

Title: MINSTED nanoscopy enters the Ångström localization range

Author: Weber, Michael, von der Emde, Henrik, Leutenegger, Marcel, Gunkel, Philip, Sambandan, Sivakumar, Khan, Taukeer A., Keller-Findeisen, Jan, Cordes, Volker C., Hell, Stefan W.

Issue&Volume: 2022-11-07

Abstract: Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the ngstrm range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the fluorophore with the low-intensity central region of a stimulated emission depletion (STED) donut beam while constantly increasing the absolute donut power. By blue-shifting the STED beam and separating fluorophores by on/off switching, individual fluorophores bound to a DNA strand are localized with σ=4.7, corresponding to a fraction of the fluorophore size, with only 2,000 detected photons. MINSTED fluorescence nanoscopy with single-digit nanometer resolution is exemplified by imaging nuclear pore complexes and the distribution of nuclear lamin in mammalian cells labeled by transient DNA hybridization. Because our experiments yield a localization precision σ=2.3, estimated for 10,000 detected photons, we anticipate that MINSTED will open up new areas of application in the study of macromolecular complexes in cells.

DOI: 10.1038/s41587-022-01519-4

Source: https://www.nature.com/articles/s41587-022-01519-4

期刊信息

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:31.864
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex