当前位置:科学网首页 > 小柯机器人 >详情
5-甲酰胞苷RNA的光促检测与测序
作者:小柯机器人 发布时间:2022/10/26 9:58:35

中科院化学所程靓团队报道了5-甲酰胞苷RNA的光促检测与测序。相关研究成果发表在2022年10月17日出版的《德国应用化学》。

5-甲酰胞苷f5C是tRNA中的表观遗传核苷酸之一。尽管f5C在基因表达调控中具有明显的重要性,但对其确切数量和位置知之甚少。

为了获取这一信息,研究人员开发了一种具有半稳定叶立德修饰的特定功能化。化学标记显示出对f5C的高选择性,并能够与类似的5-甲酰尿苷区分。研究人员实现了一种基于环化产物4,5-吡啶-2-胺-胞嘧啶-paC的荧光信号的检测策略,显示出较高的量子产率。结果表明,f5C的检测限为0.58nM。这种方法改变了f5C的氢键活性,并在测序图谱中调节了其逆转录特征。

研究发现f5C可以从具有单一碱基分辨率的tRNA片段中检测到。综上所述,这种方法代表了一种对f5C RNA敏感的、无抗体且适用的检测和测序。

附:英文原文

Title: Photo-Facilitated Detection and Sequencing of 5-Formylcytidine RNA

Author: Xiao-Yang Jin, Zu-Rui Huang, Li-Jun Xie, Li Liu, Da-Li Han, Liang Cheng

Issue&Volume: 2022-10-17

Abstract: 5-Formylcytidine f5C is one of the epigenetic nucleotides in tRNA. Despite the evident importance of f5C in gene expression regulation, little is known about its exact amount and position. To capture this information, we developed a modification-specific functionalization with a semi-stabilized ylide. The chemical labelling exhibited a high selectivity towards f5C and allowed distinction from similar 5-formyluridine. We realized a detection strategy based on the fluorescence signal of the cyclization product 4,5-pyridin-2-amine-cytidine paC, which exhibited a high quantum yield. The results clearly identified f5C with a limit of detectionat 0.58 nM. This method altered the hydrogen bonding activities of f5C and modulated of its reverse transcription signature in sequencing profile. We showed that f5C can be detected from tRNA segments with a single-base resolution. Taken together, this approach represents a sensitive, antibody-free and applicable detection and sequencing to f5C RNA.

DOI: 10.1002/anie.202210652

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202210652

 

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx