美国宾夕法尼亚大学Arjun Raj研究团队开发的ClampFISH 2.0支持快速、可扩展的扩增RNA原位检测。2022年10月24日，国际知名学术期刊《自然—方法学》发表了这一成果。

他们描述了clampFISH 2.0，一种使用倒挂锁设计的方法，可以有效地检测许多RNA物种，并以指数方式一次性放大它们的信号，同时与之前的clampFISH方法相比，节约了时间和成本。他们利用多重信号放大和顺序检测提供增加的数据量，在超过100万个细胞中检测10种不同的RNA种类。他们还展示了clampFISH 2.0在组织切片中的作用。他们期望clampFISH 2.0提供的优势将使空间转录组学的许多应用成为可能。

据介绍，RNA原位标记在可视化转录本和量化其在单细胞中的水平方面具有巨大的潜力。但在同时产生高水平的信号的同时，实现对多个RNA种类的多重检测仍然具有挑战性。

附：英文原文

Title: ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ

Author: Dardani, Ian, Emert, Benjamin L., Goyal, Yogesh, Jiang, Connie L., Kaur, Amanpreet, Lee, Jasmine, Rouhanifard, Sara H., Alicea, Gretchen M., Fane, Mitchell E., Xiao, Min, Herlyn, Meenhard, Weeraratna, Ashani T., Raj, Arjun

Issue&Volume: 2022-10-24

Abstract: RNA labeling in situ has enormous potential to visualize transcripts and quantify their levels in single cells, but it remains challenging to produce high levels of signal while also enabling multiplexed detection of multiple RNA species simultaneously. Here, we describe clampFISH 2.0, a method that uses an inverted padlock design to efficiently detect many RNA species and exponentially amplify their signals at once, while also reducing the time and cost compared with the prior clampFISH method. We leverage the increased throughput afforded by multiplexed signal amplification and sequential detection to detect 10 different RNA species in more than 1million cells. We also show that clampFISH 2.0 works in tissue sections. We expect that the advantages offered by clampFISH 2.0 will enable many applications in spatial transcriptomics.

DOI: 10.1038/s41592-022-01653-6

Source: https://www.nature.com/articles/s41592-022-01653-6