复旦大学雷祖海团队开发了用于第二近红外窗口中比率荧光探针的聚甲基胺分子平台。相关研究成果发表在2022年10月25日出版的国际学术期刊《美国化学会杂志》。

通过具有内置自校准功能的第二近红外窗口（NIR-II）中的分子比率荧光探针可视化生物分子，例如活生物体深层组织中的酶，可以直接提供有关相关病理生理过程的可靠信息，但由于NIR-II中缺乏荧光调制策略，目前尚不可行。

该文中，研究人员通过整合罗丹明6G支架和多胺来合成了一个分子平台Py-2。当Py-2的仲胺被酰化时，其最大发射波长为1010nm。基于Py-2，构建了两种分子比率NIR-II荧光探针，即硝基还原酶响应型Rap-N和ROS响应型Rap-R，并成功地在体内外进行了验证。总的来说，该研究提出了一种独特的方法来开发用于体内生物传感的比率NIR-II分子探针。

附：英文原文

Title: Polymethine Molecular Platform for Ratiometric Fluorescent Probes in the Second near-Infrared Window

Author: Qingchun Lan, Peng Yu, Kui Yan, Xiaomin Li, Fan Zhang, Zuhai Lei

Issue&Volume: October 25, 2022

Abstract: Visualizing biomolecules such as enzymes in the deep tissue of living organisms via molecular ratiometric fluorescent probes in the second near-infrared window (NIR-II) with a built-in self-calibration function can provide reliable information about relevant pathophysiological processes directly but so far is not feasible due to the lack of a fluorescence modulation strategy in the NIR-II window. Here we present a molecular platform Py-2 by integrating the rhodamine 6G scaffold and polymethine. The maximal emission wavelength of Py-2 was 1010 nm and blue-shifted to 945 nm when its secondary amine was acylated. Based on Py-2, two molecular ratiometric NIR-II fluorescent probes, nitroreductase-responsive Rap-N and ROS-responsive Rap-R, were constructed and successfully demonstrated in vitro and in vivo. Overall, this report presents a unique approach to developing ratiometric NIR-II molecular probes for in vivo biosensing.

DOI: 10.1021/jacs.2c10041

Source: https://pubs.acs.org/doi/10.1021/jacs.2c10041