研究人员重构了大肠杆菌的遗传密码结构,并创造了正交的遗传密码,限制了合成的遗传信息逃逸到自然界中。研究人员开发了正交和相互正交的水平基因转移系统,它允许使用相同遗传密码的生物体之间转移遗传信息,但限制使用不同遗传密码的生物体之间转移遗传信息。
此外,研究人员表明,将重构的密码锁定在合成生物体中,可以完全阻止移动遗传元件的入侵,这些元件携带自己的翻译因子,并成功入侵具有经典和压缩的遗传代码的生物体。
据悉,近乎普遍的遗传密码定义了基因中的密码子和蛋白质中的氨基酸之间的对应关系。
附:英文原文
Title: Refactored genetic codes enable bidirectional genetic isolation
Author: Jérme F. Zürcher, Wesley E. Robertson, Tomás Kappes, Gianluca Petris, Thomas S. Elliott, George P. C. Salmond, Jason W. Chin
Issue&Volume: 2022-10-20
Abstract: The near-universal genetic code defines the correspondence between codons in genes and amino acids in proteins. Here we refactored the structure of the genetic code in Escherichia coli and created orthogonal genetic codes which restrict the escape of synthetic genetic information into natural life. We developed orthogonal, and mutually orthogonal, horizontal gene transfer systems, which permit the transfer of genetic information between organisms that use the same genetic code, but restrict transfer of genetic information between organisms that use different genetic codes. Moreover, we showed that locking refactored codes into synthetic organisms completely blocks invasion by mobile genetic elements, which carry their own translation factors and successfully invade organisms with canonical and compressed genetic codes.
DOI: add8943
Source: https://www.science.org/doi/10.1126/science.add8943