当前位置:科学网首页 > 小柯机器人 >详情
一种灵敏、可逆的标记策略实现对细胞表面核心聚焦糖蛋白组的全局定位
作者:小柯机器人 发布时间:2022/10/22 14:40:26

中国科学院上海药物研究所文留青团队开发了一种灵敏、可逆的标记策略实现对细胞表面核心聚焦糖蛋白组的全局定位。相关研究成果于2022年10月12日发表在《德国应用化学》。

核心岩藻糖基化,即α1,6-岩藻糖附着于N-聚糖最内层的N-乙酰氨基葡萄糖(GlcNAc)残基,通过调节许多膜蛋白与肿瘤生长、侵袭、转移、预后和免疫逃避密切相关。然而,由于缺乏有效的分析方法来识别细胞表面核心岩藻糖基化糖蛋白,尤其是糖基化位点,有关其功能机制的详细信息仍基本未知。

该文中,研究人员开发了一种用于探测核心岩藻糖基化的灵敏可逆标记策略,通过该策略,位于细胞表面的核心岩藻糖化糖蛋白被高灵敏度的生物素化探针选择性标记。在亲和树脂捕获后,标记的探针可以进一步酶解。珠上无痕裂解能够通过质谱(MS)对核心岩藻糖基化糖蛋白和糖基化位点进行全局定位。核心岩藻糖基化糖蛋白组的图谱提供了对核心岩藻糖化的生物学功能的深入了解。

附:英文原文

Title: A Sensitive and Reversible Labeling Strategy Enables Global Mapping of the Core-Fucosylated Glycoproteome on Cell Surfaces

Author: Liuqing Wen, Yinping Tian, Yuqiu Wang, Hongbo Yin, Yawen Luo, Fangyu Wei, Hu Zhou

Issue&Volume: 2022-10-12

Abstract: Core fucosylation, the attachment of α1,6-fucose to the innermost N-acetylglucosamine (GlcNAc) residue of  N  -glycans, has a strong relationship with tumor growth, invasion, metastasis, prognosis, and immune evasion by regulating many membrane proteins. However, details about the functional mechanism are still largely unknown due to the lack of an effective analytical method to identify cell-surface core-fucosylated glycoproteins, and especially glycosylation sites. Here, we developed a sensitive and reversible labeling strategy for probing core fucosylation, by which core-fucosylated glycoproteins that located on cell-surface were selectively tagged by a biotinylated probe with high sensitivity. The labeled probe can be further broken enzymatically after the capture by affinity resin. The on-bead traceless cleavage allowed the global mapping of core-fucosylated glycoproteins and glycosylation sites by mass spectrometry (MS). The profile of core-fucosylated glycoproteome provides an in-depth understanding of the biological functions of core fucosylation.

DOI: 10.1002/anie.202206802

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202206802

期刊信息

Angewandte Chemie:《德国应用化学》,创刊于1887年。隶属于德国化学会,最新IF:12.959
官方网址:https://onlinelibrary.wiley.com/journal/15213773
投稿链接:https://www.editorialmanager.com/anie/default.aspx