正常和畸形人脑血管系统单细胞图谱，这一成果由美国加州大学Tomasz J. Nowakowski、DANIEL A. LIM和Adib A. Abla研究组经过不懈努力而取得。这一研究成果于2022年1月27日发表在国际学术期刊《科学》上。

研究人员分析了181,388个细胞的转录组，以确定成人脑血管系统的细胞图谱，包括具有动静脉分割和血管周围细胞多样性的内皮细胞分子特征。通过这一图谱，研究人员探究了脑动静脉畸形（年轻人中风的主要原因）的细胞和分子扰动，并确定了具有异常血管模式的病理性内皮转化和血管源性炎症的成因。在该研究中，研究人员证明了导致脑出血血管和免疫细胞之间的相互作用，为靶向血管畸形中血管生成和炎症治疗提供了潜在可能。

据悉，脑血管疾病是导致死亡和神经功能障碍的主要成因。进一步理解疾病机制和治疗策略需要对人类脑血管细胞有更深入的了解。

附：英文原文

Title: A single-cell atlas of the normal and malformed human brain vasculature

Author: Ethan A. Winkler, Chang N. Kim, Jayden M. Ross, Joseph H. Garcia, Eugene Gil, Irene Oh, Lindsay Q. Chen, David Wu, Joshua S. Catapano, Kunal Raygor, Kazim Narsinh, Helen Kim, Shantel Weinsheimer, Daniel L. Cooke, Brian P. Walcott, Michael T. Lawton, Nalin Gupta, Berislav V. Zlokovic, Edward F. Chang, Adib A. Abla, Daniel A. Lim, Tomasz J. Nowakowski

Issue&Volume: 2022-27-01

Abstract: Cerebrovascular diseases are a leading cause of death and neurologic disability. Further understanding of disease mechanisms and therapeutic strategies requires a deeper knowledge of cerebrovascular cells in humans. We profiled transcriptomes of 181,388 cells to define a cell atlas of the adult human cerebrovasculature, including endothelial cell molecular signatures with arteriovenous segmentation and expanded perivascular cell diversity. By leveraging this reference, we investigated cellular and molecular perturbations in brain arteriovenous malformations, a leading cause of stroke in young people, and identified pathologic endothelial transformations with abnormal vascular patterning and the ontology of vascularly derived inflammation. Here, we illustrate the interplay between vascular and immune cells that contributes to brain hemorrhage and catalog opportunities for targeting angiogenic and inflammatory programs in vascular malformations.

DOI: abi7377

Source: https://www.science.org/doi/10.1126/science.abi7377