福州大学李远明团队报道了使用膜锚定DNA多任务处理器在活细胞中系统地探测细胞信号。相关研究成果于2022年1月6日发表于国际一流学术期刊《德国应用化学》。

在它们自身所处环境中系统地探测相关的信号成分对于剖析复杂的细胞信号非常有意义。然而，由于缺乏通用和有效的方法，这仍然是一个挑战。

该文中，研究人员提出了一种细胞膜锚定DNA多任务处理器，作为“交通灯”，用于细胞信号转导的综合分析。通过DNA处理器的膜锚定，增强和可控地调节c-Met信号。此外，DNA处理器的多任务能力能够直接监测c-Met活性调节诱导的相关VEGF分泌。通过利用多功能适体核酸，该模块化设计的DNA多任务处理器系统地剖析了细胞表面受体如何与活细胞中的相关成分协调。因此，其为基础细胞生物学研究和精确医学应用提供了强大的化学工具。

附：英文原文

Title: Systematic Interrogation of Cellular Signaling in Live Cells using a Membrane-anchored DNA Multitasking Processor

Author: Shan Chen, Zhifei Xu, Shiwei Li, Hong Liang, Chen Zhang, Zonghua Wang, Jingying Li, Juan Li, Huanghao Yang

Issue&Volume: 2022-01-06

Abstract: Systematic interrogation of correlative signaling components in their native environment is of great interest for dissecting sophisticated cellular signaling. However, it remains a challenge because of lacking versatile and effective approaches. Herein, we propose a cell membrane-anchored DNA multitasking processor acting as a “traffic light” for integrated analyses of cellular signal transduction. Enhanced and controllable modulation of c-Met signaling was achieved by membrane-anchoring of DNA processors. Moreover, the multitasking capability of the DNA processor allowed monitoring correlative VEGF secretion induced by c-Met activity regulation directly. By exploiting versatile aptameric nucleic acids, this modular designed DNA multitasking processor dissected how cell surface receptors coordinated with related components in live cells systematically. Therefore, it provides a powerful chemical tool for both fundamental cell biology research and precision medicine applications.

DOI: 10.1002/anie.202113795

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202113795