近日，德国风湿病研究中心Chiara Romagnani团队开发出从CD34⁺造血祖细胞产生人类先天性淋巴细胞的体外平台。相关论文于2021年8月27日在线发表于国际学术期刊《免疫》。

研究人员开发了一个平台，可从脐带血和骨髓的CD34⁺造血祖细胞中可靠地产生人类先天性淋巴细胞（ILC）谱系。结果表明，一种培养条件不足以产生所有ILC亚群，相反，细胞因子和Notch信号的独特组合是必不可少的。体外产生的自然杀伤（NK）/ILC1、ILC2和ILC3的身份通过蛋白质表达、功能检测以及全局和单细胞转录组分析得到验证，并且再现了其体内ILC对应的特征和功能。这些数据将有助于ILC生物学和分化的研究。

据介绍，ILC是先天性免疫和炎症的关键影响因素，其发育和激活途径是潜在的治疗靶标。然而，人类ILC的产生还没有被系统地探索过，以前的体外研究依赖于对少数标记物或细胞因子的分析，而这些标记物或细胞因子在分配谱系身份时是不理想的。

附：英文原文

Title: An in vitro platform supports generation of human innate lymphoid cells from CD34+ hematopoietic progenitors that recapitulate ex vivo identity

Author: Daniela Carolina Hernández, Kerstin Juelke, Nils Christian Müller, Pawel Durek, Bilge Ugursu, Mir-Farzin Mashreghi, Timo Rückert, Chiara Romagnani

Issue&Volume: 2021-08-27

Abstract: Innate lymphoid cells (ILCs) are critical effectors of innate immunity and inflammation,whose development and activation pathways make for attractive therapeutic targets.However, human ILC generation has not been systematically explored, and previous in vitro investigations relied on the analysis of few markers or cytokines, which are suboptimalto assign lineage identity. Here, we developed a platform that reliably generatedhuman ILC lineages from CD34+ hematopoietic progenitors derived from cord blood and bone marrow. We showed thatone culture condition is insufficient to generate all ILC subsets, and instead, distinctcombination of cytokines and Notch signaling are essential. The identity of naturalkiller (NK)/ILC1s, ILC2s, and ILC3s generated in vitro was validated by protein expression, functional assays, and both global and single-celltranscriptome analysis, recapitulating the signatures and functions of their ex vivo ILC counterparts. These data represent a resource to aid in clarifying ILC biologyand differentiation.

DOI: 10.1016/j.immuni.2021.07.019

Source: https://www.cell.com/immunity/fulltext/S1074-7613(21)00307-1