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研究绘制出健康和疾病中人类单核细胞和巨噬细胞的跨组织单细胞图谱
作者:小柯机器人 发布时间:2021/8/8 12:11:53

新加坡A∗STAR研究所Florent Ginhoux、法国国家健康与医学研究院Charles-Antoine Dutertre等研究人员合作绘制出健康和疾病中人类单核细胞和巨噬细胞的跨组织单细胞图谱。这一研究成果于2021年7月30日在线发表在国际学术期刊《免疫》上。

研究人员表示,单核吞噬细胞(MNP)包括树突状细胞、单核细胞和巨噬细胞(MoMac),它们表现出抗微生物、稳态和免疫调节的功能。

研究人员整合了41个数据集中13个组织的178,651个MNP,生成了MNP单细胞RNA图谱(MNP-VERSE),这是一个公开的工具,用于绘制MNP和定义MNP群体的保守基因特征。接下来,研究人员生成了一个以MoMac为重点的图谱,揭示了广泛分布于多种组织的一系列特有细胞亚群。特定的病理形式在癌症和炎症中被扩增。所有肿瘤组织都含有保守的肿瘤相关巨噬细胞群。

研究人员特别关注了IL4I1+CD274(PD-L1)+IDO1+巨噬细胞,它们通过干扰素-γ(IFN-γ)和CD40/CD40L诱导的IFN刺激的单核细胞成熟,并以T细胞依赖的方式在肿瘤周边聚集。IL4I1_Mac通过色氨酸降解表现出免疫抑制特性,并促进调节性T细胞进入肿瘤。这种综合分析为统一注释和剖析健康和病理状态下的特定巨噬细胞功能提供了一个强大的在线平台。

附:英文原文

Title: Cross-tissue single-cell landscape of human monocytes and macrophages in health and disease

Author: Kevin Mulder, Amit Ashok Patel, Wan Ting Kong, Cécile Piot, Evelyn Halitzki, Garett Dunsmore, Shabnam Khalilnezhad, Sergio Erdal Irac, Agathe Dubuisson, Marion Chevrier, Xiao Meng Zhang, John Kit Chung Tam, Tony Kiat Hon Lim, Regina Men Men Wong, Rhea Pai, Ahmed Ibrahim Samir Khalil, Pierce Kah Hoe Chow, Suny Z. Wu, Ghamdan Al-Eryani, Daniel Roden, Alexander Swarbrick, Jerry Kok Yen Chan, Salvatore Albani, Lisa Derosa, Laurence Zitvogel, Ankur Sharma, Jinmiao Chen, Aymeric Silvin, Antonio Bertoletti, Camille Blériot, Charles-Antoine Dutertre, Florent Ginhoux

Issue&Volume: 2021-07-30

Abstract: Mononuclear phagocytes (MNPs) encompass dendritic cells, monocytes, and macrophages(MoMac), which exhibit antimicrobial, homeostatic, and immunoregulatory functions.We integrated 178,651 MNPs from 13 tissues across 41 datasets to generate a MNP single-cellRNA compendium (MNP-VERSE), a publicly available tool to map MNPs and define conservedgene signatures of MNP populations. Next, we generated a MoMac-focused compendiumthat revealed an array of specialized cell subsets widely distributed across multipletissues. Specific pathological forms were expanded in cancer and inflammation. Allneoplastic tissues contained conserved tumor-associated macrophage populations. Inparticular, we focused on IL4I1+CD274(PD-L1)+IDO1+ macrophages, which accumulated in the tumor periphery in a T cell-dependent mannervia interferon-γ (IFN-γ) and CD40/CD40L-induced maturation from IFN-primed monocytes.IL4I1_Macs exhibited immunosuppressive characteristics through tryptophan degradationand promoted the entry of regulatory T cell into tumors. This integrated analysisprovides a robust online-available platform for uniform annotation and dissectionof specific macrophage functions in healthy and pathological states.

DOI: 10.1016/j.immuni.2021.07.007

Source: https://www.cell.com/immunity/fulltext/S1074-7613(21)00293-4

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx