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稳定的微生物群血清抗体表位库
作者:小柯机器人 发布时间:2021/7/21 11:26:31

以色列魏茨曼科学研究所Eran Segal和Adina Weinberger团队合作取得一项新成果。他们揭示了抗人类微生物群血清抗体表位库的群体多样性和稳定性。相关论文发表在2021年7月19日出版的《自然—医学》杂志上。

他们分析了 997 名健康个体针对 244,000 种合理选择的来自肠道微生物群、致病菌和益生菌肽抗原的血清抗体反应。利用基于噬菌体展示的合成寡核苷酸文库的噬菌体免疫沉淀测序 (PhIP-Seq),他们检测了广泛的个体特异性以及针对与年龄和性别相微生物群的共同抗体反应。他们还证明,这些抗体表位库在纵向上比肠道微生物组物种丰度更稳定。相隔五年收集的 200 多人的血清样本可以准确匹配,并可作为免疫指纹。

总体而言,他们的结果表明,系统性抗体反应提供了关于肠道微生物物种组成之外的微生物群的非冗余信息层。

据了解,血清抗体可以识别病原体和共生肠道微生物群。然而,人们目前对抗体库的理解主要基于相应 B 细胞受体基因的 DNA 测序,而实际的细菌抗原靶点仍未完全表征。

附:英文原文

Title: Population-wide diversity and stability of serum antibody epitope repertoires against human microbiota

Author: Thomas Vogl, Shelley Klompus, Sigal Leviatan, Iris N. Kalka, Adina Weinberger, Cisca Wijmenga, Jingyuan Fu, Alexandra Zhernakova, Rinse K. Weersma, Eran Segal

Issue&Volume: 2021-07-19

Abstract: Serum antibodies can recognize both pathogens and commensal gut microbiota. However, our current understanding of antibody repertoires is largely based on DNA sequencing of the corresponding B-cell receptor genes, and actual bacterial antigen targets remain incompletely characterized. Here we have profiled the serum antibody responses of 997 healthy individuals against 244,000 rationally selected peptide antigens derived from gut microbiota and pathogenic and probiotic bacteria. Leveraging phage immunoprecipitation sequencing (PhIP-Seq) based on phage-displayed synthetic oligo libraries, we detect a wide breadth of individual-specific as well as shared antibody responses against microbiota that associate with age and gender. We also demonstrate that these antibody epitope repertoires are more longitudinally stable than gut microbiome species abundances. Serum samples of more than 200 individuals collected five years apart could be accurately matched and could serve as an immunologic fingerprint. Overall, our results suggest that systemic antibody responses provide a non-redundant layer of information about microbiota beyond gut microbial species composition. Phage immunoprecipitation sequencing illustrates the wide breadth of systemic microbiota-specific antibody responses, which are more longitudinally stable than gut microbiome species abundances in a cohort of healthy individuals.

DOI: 10.1038/s41591-021-01409-3

Source: https://www.nature.com/articles/s41591-021-01409-3

期刊信息

Nature Medicine:《自然—医学》,创刊于1995年。隶属于施普林格·自然出版集团,最新IF:30.641
官方网址:https://www.nature.com/nm/
投稿链接:https://mts-nmed.nature.com/cgi-bin/main.plex