美国哈佛大学James J. Collins研究团队开发出带有嵌入式合成生物传感器的可穿戴材料，可用于生物分子检测。这一研究成果于2021年6月28日在线发表在国际学术期刊《自然—生物技术》上。

研究人员报告了使用冷冻干燥、无细胞合成回路（包括基于 CRISPR 的工具）功能化的轻质、柔性基材和纺织品，这些工具可检测代谢物、化学物质和病原体核酸特征。可穿戴设备在从水性暴露事件中再水化时被激活，并通过比色变化或通过检测荧光和发光输出的光纤网络报告特定分子目标的存在。核酸的检测限可与当前的实验室方法（如定量PCR）相媲美。

研究人员展示了一种带有冻干CRISPR传感器面罩的开发，用于在室温下90 分钟内对SARS-CoV-2进行可穿戴、无创检测，除了按下按钮外无需用户干预。

据介绍，将合成生物学整合到可穿戴设备中可以扩大无创监测生理状态、疾病状态和病原体或毒素暴露的机会。然而，合成回路的运行通常需要活的工程细菌，这限制了它们在可穿戴设备中的应用。

附：英文原文

Title: Wearable materials with embedded synthetic biology sensors for biomolecule detection

Author: Peter Q. Nguyen, Luis R. Soenksen, Nina M. Donghia, Nicolaas M. Angenent-Mari, Helena de Puig, Ally Huang, Rose Lee, Shimyn Slomovic, Tommaso Galbersanini, Geoffrey Lansberry, Hani M. Sallum, Evan M. Zhao, James B. Niemi, James J. Collins

Issue&Volume: 2021-06-28

Abstract: Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limited their application in wearables. Here we report lightweight, flexible substrates and textiles functionalized with freeze-dried, cell-free synthetic circuits, including CRISPR-based tools, that detect metabolites, chemicals and pathogen nucleic acid signatures. The wearable devices are activated upon rehydration from aqueous exposure events and report the presence of specific molecular targets by colorimetric changes or via an optical fiber network that detects fluorescent and luminescent outputs. The detection limits for nucleic acids rival current laboratory methods such as quantitative PCR. We demonstrate the development of a face mask with a lyophilized CRISPR sensor for wearable, noninvasive detection of SARS-CoV-2 at room temperature within 90min, requiring no user intervention other than the press of a button.

DOI: 10.1038/s41587-021-00950-3

Source: https://www.nature.com/articles/s41587-021-00950-3